Please use this identifier to cite or link to this item: https://doi.org/10.21769/bioprotoc.2095
Title: Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
Authors: Shen, Lisha
LIANG ZHE 
YU HAO 
Keywords: Dot blot
RNA modification
m6A
Issue Date: 5-Jan-2017
Publisher: Bio-Protocol, LLC
Citation: Shen, Lisha, LIANG ZHE, YU HAO (2017-01-05). Dot Blot Analysis of N6-methyladenosine RNA Modification Levels. BIO-PROTOCOL 7 (1). ScholarBank@NUS Repository. https://doi.org/10.21769/bioprotoc.2095
Abstract: N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, such as dot blot analysis using specific m6A antibodies and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Fu et al., 2014; Shen et al., 2016). Here we describe the method for fast detection of total m6A levels in mRNA by dot blot analysis using a specific m6A antibody.
Source Title: BIO-PROTOCOL
URI: https://scholarbank.nus.edu.sg/handle/10635/168911
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2095
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