Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gku655
Title: Engineering self-contained DNA circuit for proximity recognition and localized signal amplification of target biomolecules
Authors: Ang, Yan Shan 
Yung, Lin-Yue Lanry 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
IN-SITU
Issue Date: 1-Jan-2014
Publisher: OXFORD UNIV PRESS
Citation: Ang, Yan Shan, Yung, Lin-Yue Lanry (2014-01-01). Engineering self-contained DNA circuit for proximity recognition and localized signal amplification of target biomolecules. NUCLEIC ACIDS RESEARCH 42 (14) : 9523-9530. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gku655
Abstract: Biomolecular interactions have important cellular implications, however, a simple method for the sensing of such proximal events is lacking in the current molecular toolbox. We designed a dynamic DNA circuit capable of recognizing targets in close proximity to initiate a pre-programmed signal transduction process resulting in localized signal amplification. The entire circuitwas engineered to be self-contained, i.e. it can self-assemble onto individual target molecules autonomously and form localized signal withminimal cross-talk. α-thrombin was used as a model protein to evaluate the performance of the individual modules and the overall circuit for proximity interaction under physiologically relevant buffer condition. The circuit achieved good selectivity in presence of nonspecific protein and interfering serum matrix and successfully detected for physiologically relevant α-thrombin concentration (50 nM-5 μM) in a singlemixing step without any further washing. The formation of localized signal at the interaction site can be enhanced kinetically through the control of temperature and probe concentration. This work provides a basic general framework from which other circuit modules can be adapted for the sensing of other biomolecular or cellular interaction of interest. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.
Source Title: NUCLEIC ACIDS RESEARCH
URI: https://scholarbank.nus.edu.sg/handle/10635/168697
ISSN: 03051048
13624962
DOI: 10.1093/nar/gku655
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Engineering self-contained DNA circuit for proximity recognition and localized signal amplification of target biomolecules.pdfPublished version1.95 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

11
checked on Jan 25, 2023

WEB OF SCIENCETM
Citations

11
checked on Oct 7, 2021

Page view(s)

198
checked on Jan 26, 2023

Download(s)

5
checked on Jan 26, 2023

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.