Please use this identifier to cite or link to this item: https://doi.org/10.3791/53993
Title: In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart.
Authors: Ramesh, Sindhu
ANAMIKA SINGH 
DASAN MARY CIBI 
Hausenloy DJ
Singh Manvendra Kumar 
Issue Date: 27-Apr-2016
Publisher: Journal of Visualized Experiments
Citation: Ramesh, Sindhu, ANAMIKA SINGH, DASAN MARY CIBI, Hausenloy DJ, Singh Manvendra Kumar (2016-04-27). In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart.. Journal of Visualized Experiments 2016 (110) : e53993. ScholarBank@NUS Repository. https://doi.org/10.3791/53993
Abstract: During embryogenesis, the epicardial contribution to coronary vasculature development has been very well established. Cells derived from the epicardium differentiate into smooth muscle cells, fibroblasts and endothelial cells that contribute to the formation of coronary vessels. Here we have established an in vitro culture method for embryonic epicardial cells. Using genetic labelling, we have demonstrated that the majority of the migrating cells in our explant culture are of epicardial origin. Epicardial explant cells also retain the expression of epicardial markers (Wt1 and Tbx18). Furthermore, we provide evidence that epicardial explant cells undergo epithelial to mesenchymal transition (EMT), migrate and differentiate into smooth muscle cells after Transforming growth factor beta 1 (TGF-β1) treatment in a manner indistinguishable from that of epicardial cells in vivo. In conclusion, we provide a novel method for the culture of embryonic epicardial cells, which will help to explore the role of specific genes in epicardial cell biology.
Source Title: Journal of Visualized Experiments
URI: https://scholarbank.nus.edu.sg/handle/10635/168478
ISSN: 1940-087X
DOI: 10.3791/53993
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