Please use this identifier to cite or link to this item:
Title: Regulation of antigen presentation in dendritic cells
Keywords: antigen presentation,dendritic cells,DC-CASPIC,CD74,Nitric oxide synthase,caspase
Issue Date: 22-Aug-2008
Citation: HUANG DACHUAN (2008-08-22). Regulation of antigen presentation in dendritic cells. ScholarBank@NUS Repository.
Abstract: In this project, we identified and described the function of a novel isoform of CIITA, DC-CASPIC in mitochondria in dendritic cells (DCs). DC-CASPIC inhibits the caspase-dependent degradation of NOS2 and induces NO synthesis in maturing DCs. Furthermore, DCs over-expressing DC-CASPIC enhance MHC class II, CD80 and CD86 cell surface expression and stimulate T cell proliferation. Taken together, our results strongly suggest that DC-CASPIC is one of the key molecules that regulate NO synthesis and antigen presentation during maturation of DCs.We also reported that in immature DCs, the NO donor and the over-expression of either NOS2 or NOS3 alone could induce the cell surface expression of MHC class II, CD80 and CD86 and enhance T cell proliferation in vitro. Interestingly, NOS2 interacted with CD74, and the degradation of CD74 by caspases in immature DCs was inhibited upon treatment with the NO donor.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Huang Dachuan Thesis.pdf2.91 MBAdobe PDF



Page view(s)

checked on Apr 12, 2019


checked on Apr 12, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.