Please use this identifier to cite or link to this item:
|Title:||Label-free electrochemical DNA and protein detection using ruthenium complexes and functional polyethylenedioxythiophenes||Authors:||XIE HONG||Keywords:||DNA, protein, electrochemical detection, label-free, ruthenium complexes, polyethylenedioxythiophene (PEDOT)||Issue Date:||25-Feb-2009||Citation:||XIE HONG (2009-02-25). Label-free electrochemical DNA and protein detection using ruthenium complexes and functional polyethylenedioxythiophenes. ScholarBank@NUS Repository.||Abstract:||This thesis presents our study on the development of label-free electrochemical biosensors for DNA and protein detection. In part I, we studied label-free electrochemical DNA detection using ruthenium-complexed intercalators. The electroactive intercalators have dual function, selective binding of double-stranded DNA (ds-DNA) and generation of catalytic electrochemical signals, allowing simple yet sensitive detection. In part II, we explored the use of a conducting polymer, functionalized polyethylenedioxythiophene (PEDOT), as an intrinsic transducer for label-free protein sensing. Various approaches for the synthesis of 1-D PEDOT nanostructures were studied. Label-free detection of a blood-clogging factor, thrombin, was demonstrated using aptamer-modified nanowires. In comparison with 2-D thin films, 1-D nanostructures are crucial for field effect transistor (FET) based sensing. Finally, we evaluated functional PEDOT thin films as tunable nanobiointerfaces for cell based studies, for future in-vivo applications. Specific protein adsorption and controlled cell attachment were achieved on these biologically-relevant functionalized PEDOT surfaces.||URI:||http://scholarbank.nus.edu.sg/handle/10635/16745|
|Appears in Collections:||Ph.D Theses (Open)|
Show full item record
Files in This Item:
|XieH.pdf||4.86 MB||Adobe PDF|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.