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Title: Proteome analyses of Butyrate-treated HCT-116 colorectal cancer cells
Keywords: colorectal cancer, butyrate, cell maturation, proteomics, 2-D DIGE, iTRAQ
Issue Date: 13-Feb-2009
Citation: TAN HWEE TONG (2009-02-13). Proteome analyses of Butyrate-treated HCT-116 colorectal cancer cells. ScholarBank@NUS Repository.
Abstract: It was discovered that butyrate, derived from the fermentation of fiber, is responsible for the protective effects of fiber-rich diet in colorectal cancer prevention. To better understand butyrate's chemopreventive role in this disease, we used an integrated proteomics approach to identify the proteins involved during the maturation of butyrate-treated colorectal cancer cells. Firstly, we performed 2-D DIGE of 24h butyrate-treated HCT-116 cells to identify protein targets of butyrate involved in the initiation of growth arrest and apoptosis. We showed that hnRNP A1 underwent down-regulation, cleavage and cytoplasmic retention upon butyrate treatment. Secondly, we performed quantitative proteomics using iTRAQ for a temporal study of butyrate treatment. A model was proposed to illustrate our findings which uncovered several integrated cellular processes and pathways involved in growth arrest, apoptosis, and metastasis. These signature clusters of butyrate-regulated pathways are potential targets for novel chemopreventive and therapeutic drugs for treatment of colorectal cancer.
Appears in Collections:Ph.D Theses (Open)

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