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Title: Leukotriene B4 levels in human atherosclerotic plaques and abdominal aortic aneurysms
Authors: Van Den Borne P.
Van Der Laan S.W.
Bovens S.M.
Koole D.
Kowala M.C.
Michael L.F.
Schoneveld A.H.
Van De Weg S.M.
Velema E.
De Vries J.-P.
De Borst G.J.
Moll F.L.
De Kleijn D.P.V. 
Quax P.H.A.
Hoefer I.E.
Pasterkamp G.
Keywords: leukotriene B4
abdominal aorta aneurysm
aorta reconstruction
asymptomatic disease
atherosclerotic plaque
carotid endarterectomy
clinical feature
controlled study
disease association
disease course
human cell
human tissue
major clinical study
mammary artery
protein analysis
Analysis of Variance
Aortic Aneurysm, Abdominal
Case-Control Studies
Leukotriene B4
Plaque, Atherosclerotic
Issue Date: 2014
Publisher: Public Library of Science
Citation: Van Den Borne P., Van Der Laan S.W., Bovens S.M., Koole D., Kowala M.C., Michael L.F., Schoneveld A.H., Van De Weg S.M., Velema E., De Vries J.-P., De Borst G.J., Moll F.L., De Kleijn D.P.V., Quax P.H.A., Hoefer I.E., Pasterkamp G. (2014). Leukotriene B4 levels in human atherosclerotic plaques and abdominal aortic aneurysms. PLoS ONE 9 (1) : e86522. ScholarBank@NUS Repository.
Abstract: Background: Leukotriene B4 (LTB4) has been associated with the initiation and progression of atherosclerosis and abdominal aortic aneurysm (AAA) formation. However, associations of LTB4 levels with tissue characteristics and adverse clinical outcome of advanced atherosclerosis and AAA are scarcely studied. We hypothesized that LTB4 levels are associated with a vulnerable plaque phenotype and adverse clinical outcome. Furthermore, that LTB4 levels are associated with inflammatory AAA and adverse clinical outcome. Methods: Atherosclerotic plaques and AAA specimens were selected from two independent databases for LTB4 measurements. Plaques were isolated during carotid endarterectomy from asymptomatic (n = 58) or symptomatic (n = 317) patients, classified prior to surgery. LTB4 levels were measured without prior lipid extraction and levels were corrected for protein content. LTB4 levels were related to plaque phenotype, baseline patient characteristics and clinical outcome within three years following surgery. Seven non-diseased mammary artery specimens served as controls. AAA specimens were isolated during open repair, classified as elective (n = 189), symptomatic (n = 29) or ruptured (n = 23). LTB4 levels were measured similar to the plaque measurements and were related to tissue characteristics, baseline patient characteristics and clinical outcome. Twenty-six non-diseased aortic specimens served as controls. Results: LTB4 levels corrected for protein content were not significantly associated with histological characteristics specific for vulnerable plaques or inflammatory AAA as well as clinical presentation. Moreover, it could not predict secondary manifestations independently investigated in both databases. However, LTB4 levels were significantly lower in controls compared to plaque (p = 0.025) or AAA (p = 0.017). Conclusions: LTB4 levels were not associated with a vulnerable plaque phenotype or inflammatory AAA or clinical presentation. This study does not provide supportive evidence for a role of LTB4 in atherosclerotic plaque destabilization or AAA expansion. However, these data should be interpreted with care, since LTB4 measurements were performed without prior lipid extractions. © 2014 van den Borne et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0086522
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