Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0046264
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dc.titleInterleukin 23 Promotes Hepatocellular Carcinoma Metastasis via NF-Kappa B Induced Matrix Metalloproteinase 9 Expression
dc.contributor.authorLi J.
dc.contributor.authorLau G.
dc.contributor.authorChen L.
dc.contributor.authorYuan Y.-F.
dc.contributor.authorHuang J.
dc.contributor.authorLuk J.M.
dc.contributor.authorXie D.
dc.contributor.authorGuan X.-Y.
dc.date.accessioned2020-03-31T03:03:56Z
dc.date.available2020-03-31T03:03:56Z
dc.date.issued2012
dc.identifier.citationLi J., Lau G., Chen L., Yuan Y.-F., Huang J., Luk J.M., Xie D., Guan X.-Y. (2012). Interleukin 23 Promotes Hepatocellular Carcinoma Metastasis via NF-Kappa B Induced Matrix Metalloproteinase 9 Expression. PLoS ONE 7 (9) : e46264. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0046264
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/166208
dc.description.abstractBackground: Hepatocellular carcinoma (HCC) is one of the most popular cancers in the world with poor prognosis, which often develops from chronic liver inflammatory diseases. Interleukin 23 (IL-23) is an inflammatory cytokine which is reported to play an important role in tumor development in animal model. While the function of IL-23 in HCC development remains unknown, so we investigate the role of IL-23 in HCC progression in this study. Methodology and Principal Finding: Transcript level of IL-23, interleukin17A (IL-17A) and matrix metalloproteinases 9 (MMP9) in clinical HCC samples (n = 81) was determined by qPCR. Protein expression pattern of IL-23 in primary and metastatic HCC tissues pairs (n = 49 pairs) was determined by immunohistochemistry staining. Cell migration, invasion, RNA interfering and immune blotting were used to characterize the functional and signaling mechanisms in IL-23-treated HCC. Compared with paired non-tumor tissue, higher IL-23 expression was detected in HCC tumor tissues with metastasis. Immunohistochemistry staining confirmed the high expression of IL-23 in metastasis HCC. Immune blotting demonstrated that IL-23 was highly expressed in HCC cell lines with metastasis. Functional study found that IL-23 could promote HCC cell migration and invasion. Molecular analysis revealed that IL-23 could upregulate MMP9 expression via NF-?B/p65 signaling activation and IL-17A could improve IL-23 expression in tumor cells directly via activating NF-?B/p65 signaling pathway. Conclusions: IL-23 could promote HCC metastasis by the upregulation of MMP9 expression via activating NF-?B/p65 signaling pathway. At the same time, IL-17A could further promote IL-23 expression in HCC tumor cells. © 2012 Li et al.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectgelatinase B
dc.subjectimmunoglobulin enhancer binding protein
dc.subjectinterleukin 17
dc.subjectinterleukin 23
dc.subjecttranscription factor RelA
dc.subjectadult
dc.subjectarticle
dc.subjectcell invasion
dc.subjectcell migration
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunoblotting
dc.subjectimmunohistochemistry
dc.subjectliver cell carcinoma
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmetastasis
dc.subjectpolymerase chain reaction
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectquantitative analysis
dc.subjectRNA interference
dc.subjectsignal transduction
dc.subjectCarcinoma, Hepatocellular
dc.subjectCell Movement
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectInterleukin-17
dc.subjectInterleukin-23
dc.subjectLiver Neoplasms
dc.subjectMatrix Metalloproteinase 9
dc.subjectNeoplasm Metastasis
dc.subjectNF-kappa B
dc.subjectPolymerase Chain Reaction
dc.subjectAnimalia
dc.typeArticle
dc.contributor.departmentDEPT OF ANATOMY
dc.contributor.departmentDEPT OF PHARMACOLOGY
dc.description.doi10.1371/journal.pone.0046264
dc.description.sourcetitlePLoS ONE
dc.description.volume7
dc.description.issue9
dc.description.pagee46264
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