Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0060303
Title: Giemsa-Stained Wet Mount Based Method for Reticulocyte Quantification: A Viable Alternative in Resource Limited or Malaria Endemic Settings
Authors: Lee W.-C.
Russell B. 
Lau Y.-L.
Fong M.-Y.
Chu C.
Sriprawat K.
Suwanarusk R.
Nosten F.
Renia L. 
Keywords: methylene blue
accuracy
analytic method
article
blood sampling
cell motion
cell suspension
cellular distribution
controlled study
cost effectiveness analysis
endemic disease
Giemsa stain
human
human cell
intermethod comparison
major clinical study
malaria falciparum
mixed infection
Plasmodium malariae infection
Plasmodium vivax malaria
reticulocyte count
Azure Stains
Humans
Malaria
Methylene Blue
Reticulocyte Count
Reticulocytes
Issue Date: 2013
Publisher: Public Library of Science
Citation: Lee W.-C., Russell B., Lau Y.-L., Fong M.-Y., Chu C., Sriprawat K., Suwanarusk R., Nosten F., Renia L. (2013). Giemsa-Stained Wet Mount Based Method for Reticulocyte Quantification: A Viable Alternative in Resource Limited or Malaria Endemic Settings. PLoS ONE 8 (4) : e60303. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0060303
Abstract: The quantity of circulating reticulocytes is an important indicator of erythropoietic activity in response to a wide range of haematological pathologies. While most modern laboratories use flow cytometry to quantify reticulocytes, most field laboratories still rely on 'subvital' staining. The specialist 'subvital' stains, New Methylene Blue (NMB) and Brilliant Crésyl Blue are often difficult to procure, toxic, and show inconsistencies between batches. Here we demonstrate the utility of Giemsa's stain (commonly used microbiology and parasitology) in a 'subvital' manner to provide an accurate method to visualize and count reticulocytes in blood samples from normal and malaria-infected individuals. © 2013 Lee et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/166199
ISSN: 19326203
DOI: 10.1371/journal.pone.0060303
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