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https://doi.org/10.1371/journal.pone.0079743
Title: | Analysis of two lysozyme genes and antimicrobial functions of their recombinant proteins in Asian seabass | Authors: | Fu G.H. Bai Z.Y. Xia J.H. Liu F. Liu P. Yue G.H. |
Keywords: | lysozyme recombinant protein antiinfective agent complementary DNA lysozyme recombinant protein amino acid sequence animal experiment animal tissue antibacterial activity article bacteriolysis bass big belly disease chicken type lysozyme gene controlled study DNA marker DNA sequence Escherichia coli fish disease gene expression genetic variability goose type lysozyme gene infection resistance intestine kidney Lates calcarifer liver molecular cloning muscle nonhuman nucleotide sequence Photobacterium damselae single nucleotide polymorphism spleen tissue distribution upregulation Vibrio harveyi allele animal bass classification disease resistance female gene expression profiling gene frequency genetics genotype immunology male microbiology molecular evolution molecular genetics nucleotide sequence phylogeny Alleles Amino Acid Sequence Animals Anti-Bacterial Agents Anti-Infective Agents Base Sequence Bass Disease Resistance DNA, Complementary Evolution, Molecular Female Fish Diseases Gene Expression Profiling Gene Frequency Genotype Male Molecular Sequence Data Muramidase Phylogeny Polymorphism, Single Nucleotide Recombinant Proteins |
Issue Date: | 2013 | Publisher: | Public Library of Science | Citation: | Fu G.H., Bai Z.Y., Xia J.H., Liu F., Liu P., Yue G.H. (2013). Analysis of two lysozyme genes and antimicrobial functions of their recombinant proteins in Asian seabass. PLoS ONE 8 (11) : e79743. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0079743 | Abstract: | Lysozymes are important proteins of the innate immune system for the defense against bacterial infection. We cloned and analyzed chicken-type (c-type) and goose-type (g-type) lysozymes from Asian seabass (Lates calcarifer). The deduced amino acid sequence of the c-type lysozyme contained 144 residues and possessed typical structure residues, conserved catalytic residues (Glu 50 and Asp 67 ) and a "GSTDYGIFQINS" motif. The deduced g-type lysozyme contained 187 residues and possessed a goose egg white lysozyme (GEWL) domain containing three conserved catalytic residues (Glu 71 , Asp 84 , Asp 95 ) essential for catalytic activity. Real time quantitative PCR (qRT-PCR) revealed that the two lysozyme genes were constitutively expressed in all the examined tissues. The c-type lysozyme was most abundant in liver, while the g-type lysozyme was predominantly expressed in intestine and weakly expressed in muscle. The c-type and g-type transcripts were up-regulated in the kidney, spleen and liver in response to a challenge with Vibrio harveyi. The up-regulation of the c-type lysozyme was much stronger than that of the g-type lysozyme in kidney and spleen. The recombinant proteins of the c-type and g-type lysozymes showed lytic activities against the bacterial pathogens Vibrio harveyi and Photobacterium damselae in a dosage-dependent manner. We identified single nucleotide polymorphisms (SNPs) in the two lysozyme genes. There were significant associations of these polymorphisms with resistance to the big belly disease. These results suggest that the c- and g-type genes play an important role in resistance to bacterial pathogens in fish. The SNP markers in the two genes associated with the resistance to bacterial pathogens may facilitate the selection of Asian seabass resistant to bacterial diseases. © 2013 Fu et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/166188 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0079743 |
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