Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0168596
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dc.titleMolecular evidence of transmission of influenza A/H1N1 2009 on a University Campus
dc.contributor.authorVirk R.K.
dc.contributor.authorGunalan V.
dc.contributor.authorLee H.K.
dc.contributor.authorInoue M.
dc.contributor.authorChua C.
dc.contributor.authorTan B.-H.
dc.contributor.authorTambyah P.A.
dc.date.accessioned2020-03-27T06:29:02Z
dc.date.available2020-03-27T06:29:02Z
dc.date.issued2017
dc.identifier.citationVirk R.K., Gunalan V., Lee H.K., Inoue M., Chua C., Tan B.-H., Tambyah P.A. (2017). Molecular evidence of transmission of influenza A/H1N1 2009 on a University Campus. PLoS ONE 12 (1) : 168596. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0168596
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/166028
dc.description.abstractBackground In the recent years, the data on the molecular epidemiology of influenza viruses have expanded enormously because of the availability of cutting-edge sequencing technologies. However, much of the information is from the temperate regions with few studies from tropical regions such as South-east Asia. Despite the fact that influenza has been known to transmit rapidly within semi-closed communities, such as military camps and educational institutions, data are limited from these communities. Objectives To determine the phylogeography of influenza viruses on a university campus, we examined the spatial distribution of influenza virus on the National University of Singapore (NUS) campus. Methods Consenting students from the NUS who sought medical attention at the UHC provided two nasopharyngeal swabs and demographic data. PCR was used for detection of influenza viruses. 34 full-genomes of pH1N1/09 viruses were successfully sequenced by Sanger method and concatenated using Geneious R7. Phylogenetic analysis was conducted using these 34 sequences and 1518 global sequences. Phylogeographic analysis was done using BaTS software and Association index and Fitch parsimony scores were determined. Results Integrating whole genome sequencing data with epidemiological data, we found strong evidence of influenza transmission on campus as isolates from students residing on-campus were highly similar to each other (AI, P value = 0.009; PS, P value = 0.04). There was also evidence of multiple introductions from the community. Conclusions Such data are useful in formulating pandemic preparedness plans which can use these communities as sentinel sites for detection and monitoring of emerging respiratory viral infections. © 2017 Virk et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectvirus hemagglutinin
dc.subject2009 H1N1 influenza
dc.subjectadult
dc.subjectamino acid sequence
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectgene sequence
dc.subjecthuman
dc.subjectInfluenza A virus (H1N1)
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectnucleotide sequence
dc.subjectphylogenetic tree
dc.subjectphylogeny
dc.subjectphylogeography
dc.subjectpolymerase chain reaction
dc.subjectsequence alignment
dc.subjectSingapore
dc.subjectsocial interaction
dc.subjectunindexed sequence
dc.subjectuniversity
dc.subjectvirus transmission
dc.subjectInfluenza A virus (H1N1)
dc.subjectInfluenza, Human
dc.subjectmolecular epidemiology
dc.subjectphylogeography
dc.subjecttransmission
dc.subjectuniversity
dc.subjectvirology
dc.subjectyoung adult
dc.subjectAdult
dc.subjectFemale
dc.subjectHumans
dc.subjectInfluenza A Virus, H1N1 Subtype
dc.subjectInfluenza, Human
dc.subjectMale
dc.subjectMolecular Epidemiology
dc.subjectPhylogeny
dc.subjectPhylogeography
dc.subjectSingapore
dc.subjectUniversities
dc.subjectYoung Adult
dc.typeArticle
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.contributor.departmentUNIVERSITY HEALTH CENTRE
dc.contributor.departmentSAW SWEE HOCK SCHOOL OF PUBLIC HEALTH
dc.description.doi10.1371/journal.pone.0168596
dc.description.sourcetitlePLoS ONE
dc.description.volume12
dc.description.issue1
dc.description.page168596
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