Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0170805
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dc.titleGAD1 gene expression in blood of patients with first-episode psychosis
dc.contributor.authorYee J.Y.
dc.contributor.authorNurjono M.
dc.contributor.authorTeo S.R.
dc.contributor.authorLee T.-S.
dc.contributor.authorLee J.
dc.date.accessioned2020-03-27T06:28:45Z
dc.date.available2020-03-27T06:28:45Z
dc.date.issued2017
dc.identifier.citationYee J.Y., Nurjono M., Teo S.R., Lee T.-S., Lee J. (2017). GAD1 gene expression in blood of patients with first-episode psychosis. PLoS ONE 12 (1) : e0170805. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0170805
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/166026
dc.description.abstractã-Aminobutyric acid (GABA), the primary inhibitory neurotransmitter, has often been studied in relation to its role in the pathophysiology of schizophrenia. GABA is synthesized from glutamate by glutamic acid decarboxylase (GAD), derived from two genes, GAD1 and GAD2. GAD1 is expressed as both GAD67 and GAD25 mRNA transcripts with the former reported to have a lower expression level in schizophrenia compared to healthy controls and latter was reported to be predominantly expressed fetally, suggesting a role in developmental process. In this study, GAD67 and GAD25 mRNA levels were measured by quantitative PCR (qPCR) in peripheral blood of subjects with first-episode psychosis (FEP) and from healthy controls. We observed low GAD25 and GAD67 gene expression levels in human peripheral blood. There was no difference in GAD25 and GAD67 gene expression level, and GAD25/ GAD67 ratio between patients with FEP and healthy controls. PANSS negative symptoms were associated with levels of GAD25 mRNA transcripts in patients with FEP. While the current study provides information on GAD25 and GAD67 mRNA transcript levels in whole blood of FEP patients, further correlation and validation work between brain regions, cerebrospinal fluid and peripheral blood expression profiling are required to provide a better understanding of GAD25 and GAD67. © 2017 Yee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subject4 aminobutyric acid
dc.subjectchlorpromazine
dc.subjectglutamate decarboxylase
dc.subjectglutamate decarboxylase 1
dc.subjectglutamate decarboxylase 65
dc.subjectglutamate decarboxylase 67
dc.subjectmessenger RNA
dc.subjectunclassified drug
dc.subjectglutamate decarboxylase
dc.subjectglutamate decarboxylase 1
dc.subjectadult
dc.subjectArticle
dc.subjectblood sampling
dc.subjectcerebrospinal fluid analysis
dc.subjectcontrolled study
dc.subjectdisease duration
dc.subjectdopaminergic transmission
dc.subjectfemale
dc.subjectGABAergic system
dc.subjectgene expression
dc.subjecthuman
dc.subjectlifespan
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectneurotransmission
dc.subjectpolymerase chain reaction
dc.subjectPositive and Negative Syndrome Scale
dc.subjectprediction
dc.subjectprotein expression
dc.subjectprotein synthesis
dc.subjectpsychosis
dc.subjectquantitative analysis
dc.subjectRNA isolation
dc.subjectschizophrenia
dc.subjectsignal transduction
dc.subjectsmoking
dc.subjectgenetics
dc.subjectPsychotic Disorders
dc.subjectyoung adult
dc.subjectAdult
dc.subjectFemale
dc.subjectGene Expression
dc.subjectGlutamate Decarboxylase
dc.subjectHumans
dc.subjectMale
dc.subjectPsychotic Disorders
dc.subjectYoung Adult
dc.typeArticle
dc.contributor.departmentSAW SWEE HOCK SCHOOL OF PUBLIC HEALTH
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1371/journal.pone.0170805
dc.description.sourcetitlePLoS ONE
dc.description.volume12
dc.description.issue1
dc.description.pagee0170805
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