Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0095647
Title: Unique epitopes recognized by antibodies induced in chikungunya virus-infected non-human primates: Implications for the study of immunopathology and vaccine development
Authors: Kam Y.-W.
Lee W.W.L.
Simarmata D.
Le Grand R.
Tolou H.
Merits A.
Roques P.
Ng L.F.P. 
Keywords: epitope
glycoprotein
neutralizing antibody
NSP1 protein
nsP3 protein
nsP4 protein
unclassified drug
virus protein
virus vaccine
epitope
immunoglobulin G
neutralizing antibody
protein binding
virus antibody
virus antigen
virus protein
virus vaccine
animal experiment
animal model
antibody production
antibody response
antibody specificity
antigen recognition
article
chikungunya
Chikungunya alphavirus
controlled study
drug efficacy
enzyme linked immunosorbent assay
immune response
immunoblotting
immunopathology
kinetics
Macaca
nonhuman
virus capsid
animal
cell line
chemical structure
chemistry
Chikungunya alphavirus
Chikungunya Fever
disease model
epitope mapping
human
immunology
metabolism
primate
protein conformation
serodiagnosis
Animals
Antibodies, Neutralizing
Antibodies, Viral
Antibody Specificity
Antigens, Viral
Cell Line
Chikungunya Fever
Chikungunya virus
Disease Models, Animal
Epitope Mapping
Epitopes
Epitopes, B-Lymphocyte
Humans
Immunoglobulin G
Models, Molecular
Neutralization Tests
Primates
Protein Binding
Protein Conformation
Viral Proteins
Viral Vaccines
Issue Date: 2014
Publisher: Public Library of Science
Citation: Kam Y.-W., Lee W.W.L., Simarmata D., Le Grand R., Tolou H., Merits A., Roques P., Ng L.F.P. (2014). Unique epitopes recognized by antibodies induced in chikungunya virus-infected non-human primates: Implications for the study of immunopathology and vaccine development. PLoS ONE 9 (4) : e95647. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0095647
Abstract: Chikungunya virus (CHIKV) is an Alphavirus that causes chronic and incapacitating arthralgia in humans. Although patient cohort studies have shown the production of CHIKV specific antibodies, the fine specificity of the antibody response against CHIKV is not completely defined. The macaque model of CHIKV infection was established due to limitations of clinical specimens. More importantly, its close relation to humans will allow the study of chronic infection and further identify important CHIKV targets. In this study, serum samples from CHIKV-infected macaques collected at different time-points post infection were used to characterize the antibody production pattern and kinetics. Results revealed that anti-CHIKV antibodies were neutralizing and the E2 glycoprotein, Capsid, nsP1, nsP3 and nsP4 proteins were targets of the anti-CHIKV antibody response in macaques. Furthermore, linear B-cell epitopes recognized by these anti-CHIKV antibodies were identified, and mapped to their structural localization. This characterizes the specificity of anti-CHIKV antibody response in macaques and further demonstrates the importance of the different regions in CHIKV-encoded proteins in the adaptive immune response. Information from this study provides critical knowledge that will aid in the understanding of CHIKV infection and immunity, vaccine design, and pre-clinical efficacy studies. © 2014 Kam et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165953
ISSN: 19326203
DOI: 10.1371/journal.pone.0095647
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