Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0098248
Title: A prospective study of romanian agriculture workers for zoonotic influenza infections
Authors: Coman A.
Maftei D.N.
Krueger W.S.
Heil G.L.
Chereches R.M.
Sirlincan E.
Bria P.
Dragnea C.
Kasler I.
Valentine M.A.
Gray G.C. 
Keywords: agricultural worker
antibody titer
article
avian influenza virus
blood sampling
controlled study
follow up
hemagglutination inhibition
human
influenza
influenza A
major clinical study
nonhuman
Piacenzian
prospective study
reverse transcription polymerase chain reaction
risk factor
seroepidemiology
serology
smoking
swine influenza virus
virus detection
Agricultural Workers' Diseases
animal
cohort analysis
genetics
hemagglutination inhibition test
Influenza virus A
Influenza, Human
real time polymerase chain reaction
risk
Romania
serodiagnosis
virology
Zoonoses
Agricultural Workers' Diseases
Animals
Cohort Studies
Hemagglutination Inhibition Tests
Humans
Influenza A virus
Influenza, Human
Neutralization Tests
Odds Ratio
Prospective Studies
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Romania
Seroepidemiologic Studies
Zoonoses
Issue Date: 2014
Publisher: Public Library of Science
Citation: Coman A., Maftei D.N., Krueger W.S., Heil G.L., Chereches R.M., Sirlincan E., Bria P., Dragnea C., Kasler I., Valentine M.A., Gray G.C. (2014). A prospective study of romanian agriculture workers for zoonotic influenza infections. PLoS ONE 9 (5) : e98248. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0098248
Abstract: Background: In this prospective study we sought to examine seroepidemiological evidence for acute zoonotic influenza virus infection among Romanian agricultural workers. Methods: Sera were drawn upon enrollment (2009) and again at 12 and 24 months from 312 adult agriculture workers and 51 age-group matched controls. Participants were contacted monthly for 24 months and queried regarding episodes of acute influenza-like illnesses (ILI). Cohort members meeting ILI criteria permitted respiratory swab collections as well as acute and convalescent serum collection. Serologic assays were performed against 9 avian, 3 swine, and 3 human influenza viruses. Results: During the two-year follow-up, a total of 23 ILI events were reported. Two subjects' specimens were identified as influenza A by rRT-PCR. During the follow-up period, three individuals experienced elevated microneutralization antibody titers ?1:80 against three (one each) avian influenza viruses: A/Teal/Hong Kong/w312/97(H6N1), A/Hong Kong/1073/1999(H9N2), or A/Duck/Alberta/60/ 1976(H12N5). However, none of these participants met the criteria for poultry exposure. A number of subjects demonstrated four-fold increases over time in hemagglutination inhibition (HI) assay titers for at least one of the three swine influenza viruses (SIVs); however, it seems likely that two of these three responses were due to crossreacting antibody against human influenza. Only elevated antibody titers against A/Swine/Flanders/1/1998(H3N2) lacked evidence for such confounding. In examining risk factors for elevated antibody against this SIV with multiple logistic regression, swine exposure (adjusted OR = 1.8, 95% CI 1.1-2.8) and tobacco use (adjusted OR = 1.8; 95% CI 1.1-2.9) were important predictors. Conclusions: While Romania has recently experienced multiple incursions of highly pathogenic avian influenza among domestic poultry, this cohort of Romanian agriculture workers had sparse evidence of avian influenza virus infections. In contrast, there was evidence, especially among the swine exposed participants, of infections with human and one swine H3N2 influenza virus. © 2014 Coman et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165948
ISSN: 19326203
DOI: 10.1371/journal.pone.0098248
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