Biomimetic Nanofiber/Stem Cell Composite for Skin Graft Application

Abstract

This study hypothesized that biomimetic nanofiber scaffolds (NFS) functionalized with rich attachment of bone marrow-derived mesenchymal stem cells (BM-MSCs) can promote wound healing in acute full-thickness skin wounds which are not adequately addressed by current clinical treatments. The epidermal differentiation potential of human BM-MSCs was confirmed and achieved by manipulating the biochemical, environmental and physical cues in 3D organotypic coculture. Both NFS and BM-MSCs were found to contribute to the wound healing process in acute full-thickness skin wounds in a rat model. Biodegradable electrospun NFS improved wound healing by providing a provisional matrix for cellular invasion and adhesion, collagen synthesis and deposition. BM-MSCs were found to promote epithelial edge ingrowth, participate in epidermal differentiation and collagen synthesis. This study suggests the potential of using NFS/BM-MSCs composites as skin grafts for the treatment of acute full-thickness skin wounds.