Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0184955
Title: Immune-modulatory genomic properties differentiate gut microbiota of infants with and without eczema
Authors: Oh S.
Yap G.C. 
Hong P.-Y.
Huang C.-H. 
Aw M.M. 
Shek L.P.-C. 
Liu W.-T.
Lee B.W. 
Keywords: bacterial genome
Bifidobacterium
Bifidobacterium bifidum
Bifidobacterium breve
Bifidobacterium dentium
Bifidobacterium longum
Bifidobacterium pseudocatenulatum
biosynthesis
cohort analysis
community structure
controlled study
DNA extraction
eczema
follow up
human
immunomodulation
infant
intestine flora
metagenomics
microbial diversity
sequence analysis
shotgun sequencing
tetrapyrrole biosynthesis
case control study
eczema
female
genetics
immunology
male
phylogeny
placebo
Case-Control Studies
Eczema
Female
Gastrointestinal Microbiome
Humans
Infant
Male
Phylogeny
Placebos
Issue Date: 2017
Publisher: Public Library of Science
Citation: Oh S., Yap G.C., Hong P.-Y., Huang C.-H., Aw M.M., Shek L.P.-C., Liu W.-T., Lee B.W. (2017). Immune-modulatory genomic properties differentiate gut microbiota of infants with and without eczema. PLoS ONE 12 (10) : e0184955. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0184955
Abstract: Gut microbiota play an important role in human immunological processes, potentially affecting allergic diseases such as eczema. The diversity and structure of gut microbiota in infants with eczema have been previously documented. This study aims to evaluate by comparative metagenomics differences in genetic content in gut microbiota of infants with eczema and their matched controls. Stools were collected at the age of one month old from twelve infants from an at risk birth cohort in a case control manner. Clinical follow up for atopic outcomes were carried out at the age of 12 and 24 months. Microbial genomic DNA were extracted from stool samples and used for shotgun sequencing. Comparative metagenomic analysis showed that immune-regulatory TCAAGCTTGA motifs were significantly enriched in the six healthy controls (C) communities compared to the six eczema subjects (E), with many encoded by Bifidobacterium (38% of the total motifs in the C communities). Draft genomes of five Bifidobacterium species populations (B. longum, B. bifidum, B. breve, B. dentium, and B. pseudocatenulatum) were recovered from metagenomic datasets. The B. longum BFN-121-2 genome encoded more TCAAGCTTGA motifs (4.2 copies per one million genome sequence) than other Bifidobacterium genomes. Additionally, the communities in the stool of controls (C) were also significantly enriched in functions associated with tetrapyrrole biosynthesis compared to those of eczema (E). Our results show distinct immune-modulatory genomic properties of gut microbiota in infants associated with eczema and provide new insights into potential role of gut microbiota in affecting human immune homeostasis. © 2017 Oh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165774
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0184955
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