Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0137682
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dc.titleAnoxic biodegradation of isosaccharinic acids at alkaline pH by natural microbial communities
dc.contributor.authorRout S.P.
dc.contributor.authorCharles C.J.
dc.contributor.authorDoulgeris C.
dc.contributor.authorMcCarthy A.J.
dc.contributor.authorRooks D.J.
dc.contributor.authorLoughnane J.P.
dc.contributor.authorLaws A.P.
dc.contributor.authorHumphreys P.N.
dc.date.accessioned2020-03-19T07:51:32Z
dc.date.available2020-03-19T07:51:32Z
dc.date.issued2015
dc.identifier.citationRout S.P., Charles C.J., Doulgeris C., McCarthy A.J., Rooks D.J., Loughnane J.P., Laws A.P., Humphreys P.N. (2015). Anoxic biodegradation of isosaccharinic acids at alkaline pH by natural microbial communities. PLoS ONE 10 (9) : e0137682. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0137682
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/165763
dc.description.abstractOne design concept for the long-term management of the UK's intermediate level radioactive wastes (ILW) is disposal to a cementitious geological disposal facility (GDF). Under the alkaline (10.0<pH>13.0) anoxic conditions expected within a GDF, cellulosic wastes will undergo chemical hydrolysis. The resulting cellulose degradation products (CDP) are dominated by ?- and ?-isosaccharinic acids (ISA), which present an organic carbon source that may enable subsequent microbial colonisation of a GDF. Microcosms established from neutral, near-surface sediments demonstrated complete ISA degradation under methanogenic conditions up to pH 10.0. Degradation decreased as pH increased, with ?-ISA fermentation more heavily influenced than ?-ISA. This reduction in degradation rate was accompanied by a shift in microbial population away from organisms related to Clostridium sporosphaeroides to a more diverse Clostridial community. The increase in pH to 10.0 saw an increase in detection of Alcaligenes aquatilis and a dominance of hydrogenotrophic methanogens within the Archaeal population. Methane was generated up to pH 10.0 with acetate accumulation at higher pH values reflecting a reduced detection of acetoclastic methanogens. An increase in pH to 11.0 resulted in the accumulation of ISA, the absence of methanogenesis and the loss of biomass from the system. This study is the first to demonstrate methanogenesis from ISA by near surface microbial communities not previously exposed to these compounds up to and including pH 10.0. © 2015 Rout et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjecthemicellulose
dc.subjectmethane
dc.subjectRNA 16S
dc.subjectbacterial RNA
dc.subjectisosaccharinic acid
dc.subjectradioactive waste
dc.subjectsugar acid
dc.subjectAlcaligenes
dc.subjectAlcaligenes aquatilis
dc.subjectalkalinity
dc.subjectarchaeon
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbacterium identification
dc.subjectbiomass
dc.subjectClostridium
dc.subjectClostridium sporosphaeroides
dc.subjectgene library
dc.subjectgene sequence
dc.subjectincubation time
dc.subjectMethanobacterium
dc.subjectMethanobacterium alcaliphilum
dc.subjectMethanobacterium flexile
dc.subjectMethanobacterium subterraneum
dc.subjectmicrobial colonization
dc.subjectmicrobial community
dc.subjectmicrocosm
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpH
dc.subjectphylogeny
dc.subjectsequence homology
dc.subjectstereoisomerism
dc.subjectunindexed sequence
dc.subjectbioremediation
dc.subjectchemistry
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectpH
dc.subjectprocedures
dc.subjectradioactive waste
dc.subjectsequence analysis
dc.subjectwaste management
dc.subjectArchaea
dc.subjectBiodegradation, Environmental
dc.subjectClostridium
dc.subjectGene Library
dc.subjectHydrogen-Ion Concentration
dc.subjectMethane
dc.subjectPhylogeny
dc.subjectRadioactive Waste
dc.subjectRNA, Bacterial
dc.subjectSequence Analysis, RNA
dc.subjectSugar Acids
dc.subjectWaste Management
dc.typeArticle
dc.contributor.departmentDEPT OF SURGERY
dc.description.doi10.1371/journal.pone.0137682
dc.description.sourcetitlePLoS ONE
dc.description.volume10
dc.description.issue9
dc.description.pagee0137682
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