Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0137682
Title: Anoxic biodegradation of isosaccharinic acids at alkaline pH by natural microbial communities
Authors: Rout S.P.
Charles C.J. 
Doulgeris C.
McCarthy A.J.
Rooks D.J.
Loughnane J.P.
Laws A.P.
Humphreys P.N.
Keywords: hemicellulose
methane
RNA 16S
bacterial RNA
isosaccharinic acid
radioactive waste
sugar acid
Alcaligenes
Alcaligenes aquatilis
alkalinity
archaeon
Article
bacterial gene
bacterial strain
bacterium identification
biomass
Clostridium
Clostridium sporosphaeroides
gene library
gene sequence
incubation time
Methanobacterium
Methanobacterium alcaliphilum
Methanobacterium flexile
Methanobacterium subterraneum
microbial colonization
microbial community
microcosm
nonhuman
nucleotide sequence
pH
phylogeny
sequence homology
stereoisomerism
unindexed sequence
bioremediation
chemistry
genetics
metabolism
pH
procedures
radioactive waste
sequence analysis
waste management
Archaea
Biodegradation, Environmental
Clostridium
Gene Library
Hydrogen-Ion Concentration
Methane
Phylogeny
Radioactive Waste
RNA, Bacterial
Sequence Analysis, RNA
Sugar Acids
Waste Management
Issue Date: 2015
Publisher: Public Library of Science
Citation: Rout S.P., Charles C.J., Doulgeris C., McCarthy A.J., Rooks D.J., Loughnane J.P., Laws A.P., Humphreys P.N. (2015). Anoxic biodegradation of isosaccharinic acids at alkaline pH by natural microbial communities. PLoS ONE 10 (9) : e0137682. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0137682
Abstract: One design concept for the long-term management of the UK's intermediate level radioactive wastes (ILW) is disposal to a cementitious geological disposal facility (GDF). Under the alkaline (10.013.0) anoxic conditions expected within a GDF, cellulosic wastes will undergo chemical hydrolysis. The resulting cellulose degradation products (CDP) are dominated by ?- and ?-isosaccharinic acids (ISA), which present an organic carbon source that may enable subsequent microbial colonisation of a GDF. Microcosms established from neutral, near-surface sediments demonstrated complete ISA degradation under methanogenic conditions up to pH 10.0. Degradation decreased as pH increased, with ?-ISA fermentation more heavily influenced than ?-ISA. This reduction in degradation rate was accompanied by a shift in microbial population away from organisms related to Clostridium sporosphaeroides to a more diverse Clostridial community. The increase in pH to 10.0 saw an increase in detection of Alcaligenes aquatilis and a dominance of hydrogenotrophic methanogens within the Archaeal population. Methane was generated up to pH 10.0 with acetate accumulation at higher pH values reflecting a reduced detection of acetoclastic methanogens. An increase in pH to 11.0 resulted in the accumulation of ISA, the absence of methanogenesis and the loss of biomass from the system. This study is the first to demonstrate methanogenesis from ISA by near surface microbial communities not previously exposed to these compounds up to and including pH 10.0. © 2015 Rout et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165763
ISSN: 19326203
DOI: 10.1371/journal.pone.0137682
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