Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0120672
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dc.titleEstrogens induce rapid cytoskeleton re-organization in human dermal fibroblasts via the non-classical receptor GPR30
dc.contributor.authorCarnesecchi J.
dc.contributor.authorMalbouyres M.
dc.contributor.authorDe Mets R.
dc.contributor.authorBalland M.
dc.contributor.authorBeauchef G.
dc.contributor.authorVié K.
dc.contributor.authorChamot C.
dc.contributor.authorLionnet C.
dc.contributor.authorRuggiero F.
dc.contributor.authorVanacker J.-M.
dc.date.accessioned2020-03-19T03:02:50Z
dc.date.available2020-03-19T03:02:50Z
dc.date.issued2015
dc.identifier.citationCarnesecchi J., Malbouyres M., De Mets R., Balland M., Beauchef G., Vié K., Chamot C., Lionnet C., Ruggiero F., Vanacker J.-M. (2015). Estrogens induce rapid cytoskeleton re-organization in human dermal fibroblasts via the non-classical receptor GPR30. PLoS ONE 10 (3) : e0120672. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0120672
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/165701
dc.description.abstractThe post-menopausal decrease in estrogen circulating levels results in rapid skin deterioration pointing out to a protective effect exerted by these hormones. The identity of the skin cell type responding to estrogens is unclear as are the cellular and molecular processes they elicit. Here, we reported that lack of estrogens induces rapid re-organization of the human dermal fibroblast cytoskeleton resulting in striking cell shape change. This morphological change was accompanied by a spatial re-organization of focal adhesion and a substantial reduction of their number as evidenced by vinculin and actin co-staining. Cell morphology and cytoskeleton organization was fully restored upon 17?-estradiol (E2) addition. Treatment with specific ER antagonists and cycloheximide respectively showed that the E2 acts independently of the classical Estrogen Receptors and that cell shape change is mediated by non-genomic mechanisms. E2 treatment resulted in a rapid and transient activation of ERK1/2 but not Src or PI3K. We show that human fibroblasts express the nonclassical E2 receptor GPR30 and that its agonist G-1 phenocopies the effect of E2. Inhibiting GPR30 through treatment with the G-15 antagonist or specific shRNA impaired E2 effects. Altogether, our data reveal a novel mechanism by which estrogens act on skin fibroblast by regulating cell shape through the non-classical G protein-coupled receptor GPR30 and ERK1/2 activation. © 2015 Carnesecchi et al.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectactin
dc.subjectcycloheximide
dc.subjectestradiol
dc.subjectestrogen
dc.subjectestrogen receptor
dc.subjectG protein coupled receptor 30
dc.subjectmitogen activated protein kinase 1
dc.subjectmitogen activated protein kinase 3
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein tyrosine kinase
dc.subjectshort hairpin RNA
dc.subjectvinculin
dc.subject1,3 benzodioxole derivative
dc.subject4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta(c)quinoline
dc.subjectestradiol
dc.subjectestrogen
dc.subjectestrogen receptor
dc.subjectestrogen receptor beta
dc.subjectG protein coupled receptor
dc.subjectGPER protein, human
dc.subjectMAPK1 protein, human
dc.subjectmitogen activated protein kinase 1
dc.subjectmitogen activated protein kinase 3
dc.subjectphosphatidylinositol 3 kinase
dc.subjectquinoline derivative
dc.subjectadult
dc.subjectArticle
dc.subjectcell migration
dc.subjectcell proliferation
dc.subjectcell shape
dc.subjectcell structure
dc.subjectcollagen synthesis
dc.subjectcontrolled study
dc.subjectcytoskeleton
dc.subjectcytoskeleton reorganization
dc.subjectenzyme activation
dc.subjectfemale
dc.subjectfocal adhesion
dc.subjecthuman
dc.subjecthuman cell
dc.subjectinhibition kinetics
dc.subjectprimary culture
dc.subjectprotein expression
dc.subjectsignal transduction
dc.subjectskin fibroblast
dc.subjectagonists
dc.subjectantagonists and inhibitors
dc.subjectdermis
dc.subjectdrug effects
dc.subjectfibroblast
dc.subjectmetabolism
dc.subjectAdult
dc.subjectBenzodioxoles
dc.subjectDermis
dc.subjectEstradiol
dc.subjectEstrogen Receptor beta
dc.subjectEstrogens
dc.subjectFemale
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectMAP Kinase Signaling System
dc.subjectMitogen-Activated Protein Kinase 1
dc.subjectMitogen-Activated Protein Kinase 3
dc.subjectPhosphatidylinositol 3-Kinases
dc.subjectQuinolines
dc.subjectReceptors, Estrogen
dc.subjectReceptors, G-Protein-Coupled
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.1371/journal.pone.0120672
dc.description.sourcetitlePLoS ONE
dc.description.volume10
dc.description.issue3
dc.description.pagee0120672
dc.published.statePublished
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