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Title: | SINGLE-MOLECULE DETECTION OF ARTIFICIAL AUTONOMOUS NANOWALKER USING MAGNETIC TWEEZERS | Authors: | HU XINPENG | Keywords: | Artificial nanowalker, Single-molecule,Force-assisted in-situ assembly, Magnetic tweezers, DNA nanotechnology, Chemomechanical coupling | Issue Date: | 28-Jun-2019 | Citation: | HU XINPENG (2019-06-28). SINGLE-MOLECULE DETECTION OF ARTIFICIAL AUTONOMOUS NANOWALKER USING MAGNETIC TWEEZERS. ScholarBank@NUS Repository. | Abstract: | Single-molecule mechanical characterization for artificial DNA nanowalkers, while crucial in the development of this field, is a challenge to date. In this thesis, a procedure employing magnetic tweezers to detect an autonomous non-burn-bridge DNA nanowalker, is developed. To the writer’s knowledge, it is the first time that a single artificial nanowalker is detected in its directional motion against a mechanical force. In this setup, an in-situ assembly of the walker-track system was achieved in a stepwise fashion. This is a force-assisted multi-step annealing process by stretching a single-stranded DNA template via a single paramagnetic bead, after which the same bead is switched to the walker for motility detection. Therefore, single-molecule level quantities were obtained by analyzing the stepping signals. This study establishes a single-molecule platform to characterize the mechanical output of artificial nanowalkers, and the findings add fresh insights into chemomechanical conversion at the single-molecule level. | URI: | https://scholarbank.nus.edu.sg/handle/10635/165647 |
Appears in Collections: | Ph.D Theses (Open) |
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