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https://doi.org/10.1371/journal.pone.0015532
Title: | Interplay of substrate retention and export signals in endoplasmic reticulum quality control | Authors: | Kawaguchi S. Hsu C.-L. Ng D.T.W. |
Keywords: | cycloheximide glycan glycopeptidase membrane protein protein CPY unclassified drug polysaccharide Saccharomyces cerevisiae protein article controlled study cytotoxicity endoplasmic reticulum endoplasmic reticulum stress enzyme substrate homeostasis nonhuman nuclear export signal protein degradation protein folding protein processing quality control signal transduction transport vesicle chemistry fluorescence microscopy genetics glycosylation Golgi complex metabolism mutation protein transport Saccharomyces cerevisiae signal transduction Endoplasmic Reticulum Glycosylation Golgi Apparatus Microscopy, Fluorescence Mutation Polysaccharides Protein Folding Protein Processing, Post-Translational Protein Transport Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction |
Issue Date: | 2010 | Publisher: | Public Library of Science | Citation: | Kawaguchi S., Hsu C.-L., Ng D.T.W. (2010). Interplay of substrate retention and export signals in endoplasmic reticulum quality control. PLoS ONE 5 (11) : e15532. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0015532 | Abstract: | Background: Endoplasmic reticulum (ER) quality control mechanisms are part of a comprehensive system to manage cell stress. The flux of molecules is monitored to retain folding intermediates and target misfolded molecules to ER-associated degradation (ERAD) pathways. The mechanisms of sorting remain unclear. While some proteins are retained statically, the classical model substrate CPY* is found in COPII transport vesicles, suggesting a retrieval mechanism for retention. However, its management can be even more dynamic. If ERAD is saturated under stress, excess CPY* traffics to the vacuole for degradation. These observations suggest that misfolded proteins might display different signals for their management. Methodology/Principal Findings: Here, we report the existence of a functional ER exit signal in the pro-domain of CPY*. Compromising its integrity causes ER retention through exclusion from COPII vesicles. The signal co-exists with other signals used for retention and degradation. Physiologically, the export signal is important for stress tolerance. Disabling it converts a benign protein into one that is intrinsically cytotoxic. Conclusions/Significance: These data reveal the remarkable interplay between opposing signals embedded within ERAD substrate molecules and the mechanisms that decipher them. Our findings demonstrate the diversity of mechanisms deployed for protein quality control and maintenance of protein homeostasis. © 2010 Kawaguchi et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/165597 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0015532 |
Appears in Collections: | Elements Staff Publications |
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