Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0015532
Title: Interplay of substrate retention and export signals in endoplasmic reticulum quality control
Authors: Kawaguchi S.
Hsu C.-L. 
Ng D.T.W. 
Keywords: cycloheximide
glycan
glycopeptidase
membrane protein
protein CPY
unclassified drug
polysaccharide
Saccharomyces cerevisiae protein
article
controlled study
cytotoxicity
endoplasmic reticulum
endoplasmic reticulum stress
enzyme substrate
homeostasis
nonhuman
nuclear export signal
protein degradation
protein folding
protein processing
quality control
signal transduction
transport vesicle
chemistry
fluorescence microscopy
genetics
glycosylation
Golgi complex
metabolism
mutation
protein transport
Saccharomyces cerevisiae
signal transduction
Endoplasmic Reticulum
Glycosylation
Golgi Apparatus
Microscopy, Fluorescence
Mutation
Polysaccharides
Protein Folding
Protein Processing, Post-Translational
Protein Transport
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Signal Transduction
Issue Date: 2010
Publisher: Public Library of Science
Citation: Kawaguchi S., Hsu C.-L., Ng D.T.W. (2010). Interplay of substrate retention and export signals in endoplasmic reticulum quality control. PLoS ONE 5 (11) : e15532. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0015532
Abstract: Background: Endoplasmic reticulum (ER) quality control mechanisms are part of a comprehensive system to manage cell stress. The flux of molecules is monitored to retain folding intermediates and target misfolded molecules to ER-associated degradation (ERAD) pathways. The mechanisms of sorting remain unclear. While some proteins are retained statically, the classical model substrate CPY* is found in COPII transport vesicles, suggesting a retrieval mechanism for retention. However, its management can be even more dynamic. If ERAD is saturated under stress, excess CPY* traffics to the vacuole for degradation. These observations suggest that misfolded proteins might display different signals for their management. Methodology/Principal Findings: Here, we report the existence of a functional ER exit signal in the pro-domain of CPY*. Compromising its integrity causes ER retention through exclusion from COPII vesicles. The signal co-exists with other signals used for retention and degradation. Physiologically, the export signal is important for stress tolerance. Disabling it converts a benign protein into one that is intrinsically cytotoxic. Conclusions/Significance: These data reveal the remarkable interplay between opposing signals embedded within ERAD substrate molecules and the mechanisms that decipher them. Our findings demonstrate the diversity of mechanisms deployed for protein quality control and maintenance of protein homeostasis. © 2010 Kawaguchi et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165597
ISSN: 19326203
DOI: 10.1371/journal.pone.0015532
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