Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pbio.0060256
Title: REST regulates distinct transcriptional networks in embryonic and neural stem cells
Authors: Johnson R.
Teh C.H.-L.
Kunarso G.
Kee Y.W.
Srinivasan G.
Cooper M.L.
Volta M.
Chan S.S.-L.
Lipovich L.
Pollard S.M.
Karuturi R.K.M.
Wei C.-L.
Buckley N.J.
Stanton L.W. 
Keywords: octamer transcription factor 4
RE1 silencing transcription factor
transcription factor
transcription factor NANOG
transcription factor Sox2
transcriptome
unclassified drug
RE1 silencing transcription factor
RE1-silencing transcription factor
repressor protein
animal cell
article
controlled study
embryo
embryonic stem cell
gene
genome
mouse
neural stem cell
nonhuman
phenotype
animal
binding site
cell differentiation
cell line
cell strain 3T3
chromatin immunoprecipitation
cytology
DNA microarray
fibroblast
gene expression regulation
gene regulatory network
genetics
metabolism
nerve cell
physiology
stem cell
Animals
Binding Sites
Cell Differentiation
Cell Line
Chromatin Immunoprecipitation
Embryonic Stem Cells
Fibroblasts
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Mice
Neurons
NIH 3T3 Cells
Oligonucleotide Array Sequence Analysis
Repressor Proteins
Stem Cells
Issue Date: 2008
Publisher: Public Library of Science
Citation: Johnson R., Teh C.H.-L., Kunarso G., Kee Y.W., Srinivasan G., Cooper M.L., Volta M., Chan S.S.-L., Lipovich L., Pollard S.M., Karuturi R.K.M., Wei C.-L., Buckley N.J., Stanton L.W. (2008). REST regulates distinct transcriptional networks in embryonic and neural stem cells. PLoS Biology 6 (10) : 2205-2219. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.0060256
Abstract: The maintenance of pluripotency and specification of cellular lineages during embryonic development are controlled by transcriptional regulatory networks, which coordinate specific sets of genes through both activation and repression. The transcriptional repressor RE1-silencing transcription factor (REST) plays important but distinct regulatory roles in embryonic (ESC) and neural (NSC) stem cells. We investigated how these distinct biological roles are effected at a genomic level. We present integrated, comparative genome- and transcriptome-wide analyses of transcriptional networks governed by REST in mouse ESC and NSC. The REST recruitment profile has dual components: a developmentally independent core that is common to ESC, NSC, and differentiated cells; and a large, ESC-specific set of target genes. In ESC, the REST regulatory network is highly integrated into that of pluripotency factors Oct4-Sox2-Nanog. We propose that an extensive, pluripotency-specific recruitment profile lends REST a key role in the maintenance of the ESC phenotype. © 2008 Johnson et al.
Source Title: PLoS Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/165423
ISSN: 15449173
DOI: 10.1371/journal.pbio.0060256
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