Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1000521
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dc.titleA tether for Woronin body inheritance is associated with evolutionary variation in organelle positioning
dc.contributor.authorNg S.K.
dc.contributor.authorLiu F.
dc.contributor.authorLai J.
dc.contributor.authorLow W.
dc.contributor.authorJedd G.
dc.date.accessioned2020-03-13T05:27:35Z
dc.date.available2020-03-13T05:27:35Z
dc.date.issued2009
dc.identifier.citationNg S.K., Liu F., Lai J., Low W., Jedd G. (2009). A tether for Woronin body inheritance is associated with evolutionary variation in organelle positioning. PLoS Genetics 5 (6) : e1000521. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1000521
dc.identifier.issn15537390
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/165421
dc.description.abstractEukaryotic organelles evolve to support the lifestyle of evolutionarily related organisms. In the fungi, filamentous Ascomycetes possess dense-core organelles called Woronin bodies (WBs). These organelles originate from peroxisomes and perform an adaptive function to seal septal pores in response to cellular wounding. Here, we identify Leashin, an organellar tether required for WB inheritance, and associate it with evolutionary variation in the subcellular pattern of WB distribution. In Neurospora, the leashin (lah) locus encodes two related adjacent genes. N-terminal sequences of LAH-1 bind WBs via the WB-specific membrane protein WSC, and C-terminal sequences are required for WB inheritance by cell cortex association. LAH-2 is localized to the hyphal apex and septal pore rim and plays a role in colonial growth. In most species, WBs are tethered directly to the pore rim, however, Neurospora and relatives have evolved a delocalized pattern of cortex association. Using a new method for the construction of chromosomally encoded fusion proteins, marker fusion tagging (MFT), we show that a LAH-1/LAH-2 fusion can reproduce the ancestral pattern in Neurospora. Our results identify the link between the WB and cell cortex and suggest that splitting of leashin played a key role in the adaptive evolution of organelle localization. © 2009 Ng et al.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectfungal protein
dc.subjecthybrid protein
dc.subjectleashin
dc.subjectmembrane protein
dc.subjectunclassified drug
dc.subjectWSC protein
dc.subjectfungal protein
dc.subjectamino terminal sequence
dc.subjectarticle
dc.subjectcarboxy terminal sequence
dc.subjectcell organelle
dc.subjectcellular distribution
dc.subjectcontrolled study
dc.subjectfungus growth
dc.subjectfungus hyphae
dc.subjectgene identification
dc.subjectgene locus
dc.subjectgenetic code
dc.subjectinheritance
dc.subjectmolecular evolution
dc.subjectNeurospora
dc.subjectnonhuman
dc.subjectprotein binding
dc.subjectworonin body
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectmolecular evolution
dc.subjectNeurospora crassa
dc.subjectAscomycota
dc.subjectEukaryota
dc.subjectFungi
dc.subjectNeurospora
dc.subjectEvolution, Molecular
dc.subjectFungal Proteins
dc.subjectHyphae
dc.subjectNeurospora crassa
dc.subjectOrganelles
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pgen.1000521
dc.description.sourcetitlePLoS Genetics
dc.description.volume5
dc.description.issue6
dc.description.pagee1000521
dc.published.statePublished
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