Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1006221
DC FieldValue
dc.titleFACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC
dc.contributor.authorCharles Richard J.L.
dc.contributor.authorShukla M.S.
dc.contributor.authorMenoni H.
dc.contributor.authorOuararhni K.
dc.contributor.authorLone I.N.
dc.contributor.authorRoulland Y.
dc.contributor.authorPapin C.
dc.contributor.authorBen Simon E.
dc.contributor.authorKundu T.
dc.contributor.authorHamiche A.
dc.contributor.authorAngelov D.
dc.contributor.authorDimitrov S.
dc.date.accessioned2020-03-13T05:23:30Z
dc.date.available2020-03-13T05:23:30Z
dc.date.issued2016
dc.identifier.citationCharles Richard J.L., Shukla M.S., Menoni H., Ouararhni K., Lone I.N., Roulland Y., Papin C., Ben Simon E., Kundu T., Hamiche A., Angelov D., Dimitrov S. (2016). FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. PLoS Genetics 12 (7) : e1006221. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1006221
dc.identifier.issn15537390
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/165384
dc.description.abstractFACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER. © 2016 Charles Richard et al.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectnucleoplasmin
dc.subjecturacil DNA glycosidase
dc.subjectchromatin
dc.subjectDNA binding protein
dc.subjecthigh mobility group protein
dc.subjecthistone
dc.subjectnonhistone protein
dc.subjectnucleosome
dc.subjectRSC complex, S cerevisiae
dc.subjectSaccharomyces cerevisiae protein
dc.subjectSSRP1 protein, human
dc.subjectSWI-SNF-B chromatin-remodeling complex
dc.subjecttranscription elongation factor
dc.subjecttranscription factor
dc.subjecturacil
dc.subjectantibody affinity
dc.subjectArticle
dc.subjectcentrifugation
dc.subjectchromatin assembly and disassembly
dc.subjectcontrolled study
dc.subjectdissociation
dc.subjectDNA histone interaction
dc.subjectDNA repair
dc.subjectexcision repair
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthydrolysis
dc.subjectimmunoprecipitation
dc.subjectin vivo study
dc.subjectmass spectrometry
dc.subjectmolecular docking
dc.subjectmolecular dynamics
dc.subjectnucleosome
dc.subjectoxidative stress
dc.subjectprotein analysis
dc.subjectprotein domain
dc.subjectprotein footprinting
dc.subjectprotein function
dc.subjectprotein protein interaction
dc.subjectprotein purification
dc.subjectWestern blotting
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectchromatin
dc.subjectDNA damage
dc.subjectgenetic transcription
dc.subjectgenetics
dc.subjectHeLa cell line
dc.subjectmetabolism
dc.subjectSaccharomyces cerevisiae
dc.subjectXenopus laevis
dc.subjectAnimals
dc.subjectChromatin
dc.subjectChromatin Assembly and Disassembly
dc.subjectChromosomal Proteins, Non-Histone
dc.subjectDNA Damage
dc.subjectDNA Repair
dc.subjectDNA-Binding Proteins
dc.subjectHeLa Cells
dc.subjectHigh Mobility Group Proteins
dc.subjectHistones
dc.subjectHumans
dc.subjectNucleosomes
dc.subjectOxidative Stress
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectTranscription Factors
dc.subjectTranscription, Genetic
dc.subjectTranscriptional Elongation Factors
dc.subjectUracil
dc.subjectXenopus laevis
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.description.doi10.1371/journal.pgen.1006221
dc.description.sourcetitlePLoS Genetics
dc.description.volume12
dc.description.issue7
dc.description.pagee1006221
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pgen_1006221.pdf5.36 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

19
checked on Nov 28, 2021

WEB OF SCIENCETM
Citations

14
checked on Oct 5, 2021

Page view(s)

139
checked on Nov 18, 2021

Download(s)

1
checked on Nov 18, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.