Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1006221
Title: FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC
Authors: Charles Richard J.L. 
Shukla M.S.
Menoni H.
Ouararhni K.
Lone I.N.
Roulland Y.
Papin C.
Ben Simon E.
Kundu T.
Hamiche A.
Angelov D.
Dimitrov S.
Keywords: nucleoplasmin
uracil DNA glycosidase
chromatin
DNA binding protein
high mobility group protein
histone
nonhistone protein
nucleosome
RSC complex, S cerevisiae
Saccharomyces cerevisiae protein
SSRP1 protein, human
SWI-SNF-B chromatin-remodeling complex
transcription elongation factor
transcription factor
uracil
antibody affinity
Article
centrifugation
chromatin assembly and disassembly
controlled study
dissociation
DNA histone interaction
DNA repair
excision repair
human
human cell
hydrolysis
immunoprecipitation
in vivo study
mass spectrometry
molecular docking
molecular dynamics
nucleosome
oxidative stress
protein analysis
protein domain
protein footprinting
protein function
protein protein interaction
protein purification
Western blotting
animal
biosynthesis
chromatin
DNA damage
genetic transcription
genetics
HeLa cell line
metabolism
Saccharomyces cerevisiae
Xenopus laevis
Animals
Chromatin
Chromatin Assembly and Disassembly
Chromosomal Proteins, Non-Histone
DNA Damage
DNA Repair
DNA-Binding Proteins
HeLa Cells
High Mobility Group Proteins
Histones
Humans
Nucleosomes
Oxidative Stress
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Transcription, Genetic
Transcriptional Elongation Factors
Uracil
Xenopus laevis
Issue Date: 2016
Publisher: Public Library of Science
Citation: Charles Richard J.L., Shukla M.S., Menoni H., Ouararhni K., Lone I.N., Roulland Y., Papin C., Ben Simon E., Kundu T., Hamiche A., Angelov D., Dimitrov S. (2016). FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. PLoS Genetics 12 (7) : e1006221. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1006221
Abstract: FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER. © 2016 Charles Richard et al.
Source Title: PLoS Genetics
URI: https://scholarbank.nus.edu.sg/handle/10635/165384
ISSN: 15537390
DOI: 10.1371/journal.pgen.1006221
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