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Title: Molecular characterization and developmental expression patterns of the zebrafish twist gene family
Keywords: zebrafish, twist, expression, phylogeny, synteny, orthlogy
Issue Date: 26-Nov-2009
Citation: YEO GARE HOON (YANG YAYUN) (2009-11-26). Molecular characterization and developmental expression patterns of the zebrafish twist gene family. ScholarBank@NUS Repository.
Abstract: Four members of the twist gene family (twist1a, 1b, 2 and 3) are found in the zebrafish, and they are thought to have arisen through three rounds of gene duplication, two of which occurred prior to the tetrapod-fish split. Phylogenetic analysis groups most of the vertebrate Twist1 peptides into clade I, except for the Twist1b proteins of the acanthopterygian fish (medaka, pufferfish, stickleback), which clustered within clade III. Paralogies and orthologies among the zebrafish, medaka, and human twist genes were determined using comparative synteny analysis of the chromosomal regions flanking these genes. Comparative nucleotide substitution analyses also revealed a faster rate of nucleotide mutation/substitution in the acanthopterygian twist1b compared to the zebrafish twist1b, thus accounting for their anomalous phylogenetic clustering. Based on these analyses, a model for the evolutionary history of the twist genes has been reconstructed. I observed minimal expression overlap among the four twist genes using unique 3b UTR sequences for riboprobes, suggesting that despite their significant peptide similarity, their regulatory controls have diverged considerably, with minimal functional redundancy between them.
Appears in Collections:Master's Theses (Open)

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