Please use this identifier to cite or link to this item:
https://doi.org/10.1080/15548627.2015.1061849
DC Field | Value | |
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dc.title | Thyroid hormone induction of mitochondrial activity is coupled to mitophagy via ROS-AMPKULK1 signaling | |
dc.contributor.author | Sinha R.A. | |
dc.contributor.author | Singh B.K. | |
dc.contributor.author | Zhou J. | |
dc.contributor.author | Wu Y. | |
dc.contributor.author | Farah B.L. | |
dc.contributor.author | Ohba K. | |
dc.contributor.author | Lesmana R. | |
dc.contributor.author | Gooding J. | |
dc.contributor.author | Bay B.-H. | |
dc.contributor.author | Yen P.M. | |
dc.date.accessioned | 2020-01-22T04:40:35Z | |
dc.date.available | 2020-01-22T04:40:35Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Sinha R.A., Singh B.K., Zhou J., Wu Y., Farah B.L., Ohba K., Lesmana R., Gooding J., Bay B.-H., Yen P.M. (2015). Thyroid hormone induction of mitochondrial activity is coupled to mitophagy via ROS-AMPKULK1 signaling. Autophagy 11 (8) : 1341-1357. ScholarBank@NUS Repository. https://doi.org/10.1080/15548627.2015.1061849 | |
dc.identifier.issn | 15548627 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/163974 | |
dc.description.abstract | Currently, there is limited understanding about hormonal regulation of mitochondrial turnover. Thyroid hormone (T3) increases oxidative phosphorylation (OXPHOS), which generates reactive oxygen species (ROS) that damage mitochondria. However, the mechanism for maintenance of mitochondrial activity and quality control by this hormone is not known. Here, we used both in vitro and in vivo hepatic cell models to demonstrate that induction of mitophagy by T3 is coupled to oxidative phosphorylation and ROS production. We show that T3 induction of ROS activates CAMKK2 (calcium/calmodulin-dependent protein kinase kinase 2, b) mediated phosphorylation of PRKAA1/AMPK (50 AMPactivated protein kinase), which in turn phosphorylates ULK1 (unc-51 like autophagy activating kinase 1) leading to its mitochondrial recruitment and initiation of mitophagy. Furthermore, loss of ULK1 in T3-treated cells impairs both mitophagy as well as OXPHOS without affecting T3 induced general autophagy/lipophagy. These findings demonstrate a novel ROS-AMPK-ULK1 mechanism that couples T3-induced mitochondrial turnover with activity, wherein mitophagy is necessary not only for removing damaged mitochondria but also for sustaining efficient OXPHOS. ? 2015, Taylor & Francis Group, LLC. | |
dc.publisher | Taylor and Francis Inc. | |
dc.subject | Autophagy | |
dc.subject | Liver | |
dc.subject | Mitochondria | |
dc.subject | Mitophagy | |
dc.subject | PRKAA1/AMPK | |
dc.subject | ROS | |
dc.subject | T3 | |
dc.subject | ULK1 | |
dc.type | Article | |
dc.contributor.department | ANATOMY | |
dc.contributor.department | DEAN'S OFFICE (DUKE-NUS MEDICAL SCHOOL) | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1080/15548627.2015.1061849 | |
dc.description.sourcetitle | Autophagy | |
dc.description.volume | 11 | |
dc.description.issue | 8 | |
dc.description.page | 1341-1357 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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kaup-11-08-1061849.pdf | 1.89 MB | Adobe PDF | CLOSED | Published |
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