Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163922
DC FieldValue
dc.titleAltered gene expression with abnormal patterning of the telencephalon in embryos of diabetic Albino Swiss mice
dc.contributor.authorLiao, DM
dc.contributor.authorNg, YK
dc.contributor.authorTay, SSW
dc.contributor.authorLing, EA
dc.contributor.authorDheen, ST
dc.date.accessioned2020-01-20T06:42:28Z
dc.date.available2020-01-20T06:42:28Z
dc.date.issued2004-03-01
dc.identifier.citationLiao, DM, Ng, YK, Tay, SSW, Ling, EA, Dheen, ST (2004-03-01). Altered gene expression with abnormal patterning of the telencephalon in embryos of diabetic Albino Swiss mice. DIABETOLOGIA 47 (3) : 523-531. ScholarBank@NUS Repository.
dc.identifier.issn0012186X
dc.identifier.issn14320428
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/163922
dc.description.abstractAims/hypothesis. Several studies have shown that maternal diabetes increases the risk of congenital malformations in various organ systems including the neural tube. The present study analysed molecular and morphological changes in the forebrain of embryos from diabetic Albino Swiss mice. Methods. Maternal diabetes-induced morphological changes in the forebrain were examined histologically. Cell proliferation index was assayed by BrdU labelling. In situ hybridisation and quantitative real-time PCR were used to analyse the expression of genes coding for sonic hedgehog (Shh), Nkx2.1, brain factor-1 (BF-1) and bone morphogenetic protein-4 (Bmp4) that control forebrain patterning. Results. There were no distinguishable abnormalities in the forebrain of embryos from diabetic pregnancies on embryonic day 0.5. At embryonic day 11.5, embryos of diabetic pregnancies displayed a fusion and thickening of the ventral telencephalic neuroepithelium and a partial absence of the dorsal telencephalon, indicating a severe patterning defect in the dorsoventral axis of the telencephalon. The cell proliferation index was also higher in the ventral telencephalon of these embryos. Molecular analyses indicated that expression of Shh, Nkx2.1 and BF-1 was increased and their expression domains expanded dorsally in the ventral telencephalon in embryos of diabetic mice at embryonic day 11.5. The expression of Bmp4 was reduced in the dorsal forebrain of these embryos. At embryonic day 8.5, only Shh expression was increased. Conclusions/interpretation. Altered expression of various genes involved in dorsoventral patterning of the forebrain is associated with forebrain malformations in embryos of diabetic mice.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s00125-004-1351-5
dc.language.isoen
dc.publisherSPRINGER
dc.sourceElements
dc.subjectBF-1
dc.subjectBmp4
dc.subjectForebrain
dc.subjectMaternal diabetes
dc.subjectNeural tube
dc.subjectNkx2.1
dc.subjectShh
dc.subjectTelencephalon
dc.typeArticle
dc.date.updated2020-01-17T07:56:50Z
dc.contributor.departmentDEPT OF ANATOMY
dc.description.sourcetitleDIABETOLOGIA
dc.description.volume47
dc.description.issue3
dc.description.page523-531
dc.description.codenDBTGA
dc.identifier.isiut000220563100020
dc.description.placeGERMANY
dc.published.statePublished
dc.grant.idR-181-000-038-112
dc.grant.fundingagencyNational University of Singapore
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2004_Liao et al_Diabetolgia.pdf401.75 kBAdobe PDF

OPEN

PublishedView/Download

WEB OF SCIENCETM
Citations

28
checked on Oct 5, 2021

Page view(s)

182
checked on Jan 27, 2022

Download(s)

2
checked on Jan 27, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.