Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/163909
DC Field | Value | |
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dc.title | Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells | |
dc.contributor.author | Cao, Qiong | |
dc.contributor.author | Lu, Jia | |
dc.contributor.author | Kaur, Charanjit | |
dc.contributor.author | Sivakumar, Viswanathan | |
dc.contributor.author | Li, Fan | |
dc.contributor.author | Cheah, Pike See | |
dc.contributor.author | Dheen, S Thameem | |
dc.contributor.author | Ling, Eng-Ang | |
dc.date.accessioned | 2020-01-20T06:40:11Z | |
dc.date.available | 2020-01-20T06:40:11Z | |
dc.date.issued | 2008-08-15 | |
dc.identifier.citation | Cao, Qiong, Lu, Jia, Kaur, Charanjit, Sivakumar, Viswanathan, Li, Fan, Cheah, Pike See, Dheen, S Thameem, Ling, Eng-Ang (2008-08-15). Expression of Notch-1 receptor and its ligands Jagged-1 and Delta-1 in amoeboid microglia in postnatal rat brain and murine BV-2 cells. GLIA 56 (11) : 1224-1237. ScholarBank@NUS Repository. | |
dc.identifier.issn | 0894-1491 | |
dc.identifier.issn | 1098-1136 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/163909 | |
dc.description.abstract | Notch-1 receptor signaling pathway is involved in neuronal and glial differentiation. Its involvement in microglial functions, however, has remained elusive. This study reports the localization of Notch-1 receptor immunoreactivity in the amoeboid microglial cells (AMC) in the postnatal rat brain. By immunofluorescence, Notch-1 receptor was colocalized with its ligands, Jagged-1 and Delta-1, in the AMC in the corpus callosum and subventricular zone. Notch-1 immunopositive cells were confirmed to be microglia labeled by OX42 and lectin. Immunoexpression of Notch-1 receptor was progressively reduced with age. Western blot analysis showed that Notch-1 protein level in the corpus callosum in which the AMC were heavily populated was concomitantly decreased. In postnatal rats challenged with lipopolysaccharide (LPS), Notch-1 receptor immunofluorescence in AMC was noticeably enhanced. Furthermore, Notch-1 protein level in the corpus callosum was increased as revealed by Western blotting analysis. In primary microglial culture treated with LPS, mRNA expression of Notch-1 and its ligand Jagged-1 was upregulated but that of Delta-1 was reduced. The expression pattern of Notch-1 and its ligands was confirmed in murine BV-2 cells. Furthermore, Notch-1 neutralization with its antibody reduced its protein expression. More importantly, neutralization of Notch-1 concomitantly suppressed the mRNA expression of IL-6, IL-1, M-CSF, and iNOS; TNF-α, mRNA expression, however, was enhanced. Western blot confirmed the changes of protein level of the above except for IL-6, which remained relatively unaltered. It is concluded that Notch-1 signaling in the AMC and LPS-activated microglia/BV-2 cells modulates the expression of proinflammatory cytokines and nitric oxide. © 2008 Wiley-Liss, Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/glia.20692 | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.source | Elements | |
dc.subject | Cytokines | |
dc.subject | Ligands | |
dc.subject | Microglia | |
dc.subject | Nitric oxide synthase | |
dc.subject | Notch-1 | |
dc.type | Article | |
dc.date.updated | 2020-01-17T07:42:48Z | |
dc.contributor.department | ANATOMY | |
dc.description.sourcetitle | GLIA | |
dc.description.volume | 56 | |
dc.description.issue | 11 | |
dc.description.page | 1224-1237 | |
dc.description.coden | GLIAE | |
dc.identifier.isiut | 000257903900007 | |
dc.description.place | United States | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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2008_Cao et al_Glia.pdf | 1.21 MB | Adobe PDF | CLOSED | Published |
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