Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163908
Title: Cardiac Malformations Are Associated with Altered Expression of Vascular Endothelial Growth Factor and Endothelial Nitric Oxide Synthase Genes in Embryos of Diabetic Mice
Authors: Kumar, Srinivasan Dinesh 
Yong, Sook-Kwin
Dheen, S Thameem 
Bay, Boon-Huat 
Tay, Samuel Sam-Wah 
Keywords: Science & Technology
Life Sciences & Biomedicine
Medicine, Research & Experimental
Research & Experimental Medicine
maternal diabetes
developing heart
H9C2 cell line
VEGF
eNOS
mice
ENDOCARDIAL CUSHION FORMATION
CONGENITAL HEART-DEFECTS
OXIDATIVE STRESS
EARLY-PREGNANCY
OUTFLOW TRACT
RAT EMBRYOS
MOUSE HEART
GLUCOSE
INFANTS
MOTHERS
Issue Date: 1-Nov-2008
Publisher: SAGE PUBLICATIONS LTD
Citation: Kumar, Srinivasan Dinesh, Yong, Sook-Kwin, Dheen, S Thameem, Bay, Boon-Huat, Tay, Samuel Sam-Wah (2008-11-01). Cardiac Malformations Are Associated with Altered Expression of Vascular Endothelial Growth Factor and Endothelial Nitric Oxide Synthase Genes in Embryos of Diabetic Mice. EXPERIMENTAL BIOLOGY AND MEDICINE 233 (11) : 1421-1432. ScholarBank@NUS Repository.
Abstract: The aim of this study was to investigate the role of nitric oxide (NO), and the expression of endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) genes in developing hearts at embryonic day 13.5 of embryos from diabetic mice. The protein and mRNA expression levels of eNOS and VEGF were significantly altered in the developing hearts of embryos from diabetic mice. The NO level was significantly decreased, whereas the VEGF concentration was significantly increased in the developing hearts of the embryos from diabetic mice. In vitro study showed a significant reduction in eNOS expression and cell proliferation in cardiac myoblast cells exposed to high glucose concentrations. Further, high glucose induced apoptosis in myoblast cells. Ultrastructural changes characteristics of apoptosis, including cell blebbing, aggregation of ribosomes and vacuoles in the cytoplasm were also evident in myoblast cells exposed to high glucose. It is suggested that hyperglycemia alters the expression of eNOS and VEGF genes that are involved in the regulation of cell growth and vasculogenesis, thereby contributing to the cardiac malformations seen in embryos from diabetic mice. Copyright © 2008 by the Society for Experimental Biology and Medicine.
Source Title: EXPERIMENTAL BIOLOGY AND MEDICINE
URI: https://scholarbank.nus.edu.sg/handle/10635/163908
ISSN: 15353702
15353699
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