Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163907
Title: Global Gene Expression Analysis of Cranial Neural Tubes in Embryos of Diabetic Mice
Authors: Jiang, Boran 
Kumar, S Dinesh 
Loh, Wan Ting 
Manikandan, J 
Ling, Eng-Ang 
Tay, Samuel SW 
Dheen, S Thameem 
Keywords: Brain development
Embryo
Gene expression
Malformation
Maternal diabetes
Microarray
Issue Date: 1-Dec-2008
Publisher: Wiley
Citation: Jiang, Boran, Kumar, S Dinesh, Loh, Wan Ting, Manikandan, J, Ling, Eng-Ang, Tay, Samuel SW, Dheen, S Thameem (2008-12-01). Global Gene Expression Analysis of Cranial Neural Tubes in Embryos of Diabetic Mice. Journal of Neuroscience Research 86 (16) : 3481-3493. ScholarBank@NUS Repository.
Abstract: Maternal diabetes causes congenital malformations in various organs including the neural tube in fetuses. In this study, we have analyzed the differential gene expression profiling in the cranial neural tube of embryos from diabetic and control mice by using the oligonucleotide microarray. Expression patterns of genes and proteins that are differentially expressed in the cranial neural tube were further examined by the real-time reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunohistochemistry. Proliferation index and apoptosis were examined by BrdU (5-bromo-2-deoxyuridine) labeling and TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) assay, respectively. Embryos (E11.5) of diabetic pregnancies displayed distortion in neuroepithelia of the cranial neural tube. Microarray analysis revealed that a total of 390 genes exhibited more than twofold changes in expression level in the cranial neural tube of embryos from diabetic mice. Several genes involving apoptosis, proliferation, migration, and differentiation of neurons in the cranial neural tube were differentially expressed in embryos of diabetic pregnancy. In addition, maternal diabetes perturbed the development of choroid plexus and ventricular systems and reduced the production of proteins such as Ttr and Igf2 in the developing brain, indicating that these changes could impair the survival and proliferation of neuroepithelial cells and neurogenesis in embryos of diabetic mice. It is concluded that altered expression of a variety of genes involved in brain development is associated with cranial neural tube dysmorphogenesis that may subsequently contribute to intellectual impairment of the offspring of a diabetic mother. © 2008 Wiley-Liss, Inc.
Source Title: Journal of Neuroscience Research
URI: https://scholarbank.nus.edu.sg/handle/10635/163907
ISSN: 0360-4012
1097-4547
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