Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.jpclett.8b03465
Title: Linearization and Labeling of Single-Stranded DNA for Optical Sequence Analysis
Authors: Basak, Rajib 
Liu, Fan 
Qureshi, Sarfraz
Gupta, Neelima 
Zhang, Ce
de Vries, Renko
van Kan, Jeroen A 
Dheen, S Thameem 
van der Maarel, Johan RC 
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Atomic, Molecular & Chemical
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
BINDING
MOLECULES
PLATFORM
POLYMER
LENGTH
YOYO
DYES
Issue Date: 7-Feb-2019
Publisher: AMER CHEMICAL SOC
Citation: Basak, Rajib, Liu, Fan, Qureshi, Sarfraz, Gupta, Neelima, Zhang, Ce, de Vries, Renko, van Kan, Jeroen A, Dheen, S Thameem, van der Maarel, Johan RC (2019-02-07). Linearization and Labeling of Single-Stranded DNA for Optical Sequence Analysis. JOURNAL OF PHYSICAL CHEMISTRY LETTERS 10 (3) : 316-321. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.jpclett.8b03465
Abstract: Copyright © 2019 American Chemical Society. Genetic profiling would benefit from linearization of ssDNA through the exposure of the unpaired bases to gene-targeting probes. This is compromised by ssDNA's high flexibility and tendency to form self-annealed structures. Here, we demonstrate that self-annealing can be avoided through controlled coating with a cationic-neutral diblock polypeptide copolymer. Coating does not preclude site-specific binding of fluorescence labeled oligonucleotides. Bottlebrush-coated ssDNA can be linearized by confinement inside a nanochannel or molecular combing. A stretch of 0.32 nm per nucleotide is achieved inside a channel with a cross-section of 100 nm and a 2-fold excess of polypeptide with respect to DNA charge. With combing, the complexes are stretched to a similar extent. Atomic force microscopy of dried complexes on silica revealed that the contour and persistence lengths are close to those of dsDNA in the B-form. Labeling is based on hybridization and not limited by restriction enzymes. Enzyme-free labeling offers new opportunities for the detection of specific sequences. ©
Source Title: JOURNAL OF PHYSICAL CHEMISTRY LETTERS
URI: https://scholarbank.nus.edu.sg/handle/10635/163875
ISSN: 19487185
19487185
DOI: 10.1021/acs.jpclett.8b03465
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