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dc.titleTargeted delivery of Doxorubicin conjugated to Folic acid and Vitamin E D-a-Tocopheryl Polyethylene glycol succinate (TPGS)
dc.contributor.authorANBHARASI VANANGAMUDI
dc.identifier.citationANBHARASI VANANGAMUDI (2009-07-28). Targeted delivery of Doxorubicin conjugated to Folic acid and Vitamin E D-a-Tocopheryl Polyethylene glycol succinate (TPGS). ScholarBank@NUS Repository.
dc.description.abstractThis research developed a prodrug strategy to conjugate DOX to d-N1-tocopheryl polyethylene glycol succinate (TPGS) and folic acid (FOL) for targeted chemotherapy to enhance the therapeutic effects and reduce the side effects of the drug. We synthesized 2 conjugates, TPGS-DOX and TPGS-DOX-FOL to quantitatively evaluate the advantages of TPGS conjugation and FOL conjugation through passive and active targeting effects. The successful conjugation was confirmed by 1H NMR and FTIR. The in vitro drug release were found pH dependent, which is in favor of cancer treatment. The in vitro cellular uptake and cytotoxicity were evaluated with MCF-7 breast cancer cells. It was found that the cellular uptake of DOX increased 15.2% by TPGS conjugation and further 6.3% by FOL conjugation after 0.5 hour cell culture. The IC50 after 24 hour cell culture with MCF-7 cancer cells showed that TPGS-DOX conjugate could be 1.19-fold effective vs DOX and that TPGS-DOX-FOL could be 38.6-fold effective than TPGS-DOX and thus 45.0- fold more effective vs DOX. In vivo experiment showed that the half-life of TPGS-DOX and TPGS-DOX-FOL were increased 3.79 fold and 3.9 fold than the free DOX, and the area-under-the-curve (AUC) were increased 19.2 fold and 14.5 fold than the DOX, respectively. The biodistribution data showed that TPGS-DOX and TPGS-DOX-FOL significantly lowered drug accumulation in the heart, thereby reducing the cardiotoxicity, which is the main side effect of the DOX. Also, TPGS-DOX can limit, and TPGS-DOX-FOL can further deduce, the gastrointestinal side effect of the drug.
dc.subjectAnticancer Drugs; Cancer Chemotherapy; Prodrugs; Nanomedicine; Receptor Mediated Endocytosis (RME); Multidrug Resistance (MDR)
dc.contributor.supervisorFENG SI-SHEN
dc.contributor.supervisorHO GHIM WEI
dc.description.degreeconferredMASTER OF ENGINEERING
Appears in Collections:Master's Theses (Open)

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