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Title: Genetic and genomic approaches to study liver development in Zebrafish
Authors: GUO LIN
Keywords: zebrafish, liver, development, genetic, genomic
Issue Date: 4-Apr-2007
Citation: GUO LIN (2007-04-04). Genetic and genomic approaches to study liver development in Zebrafish. ScholarBank@NUS Repository.
Abstract: The liver is one of the most important organs in human body. However, the development of the liver has not been well studied. In this project we utilized zebrafish, Danio rerio, as a model system to find genes which are involved in liver organogenesis via anatomical, genetic and genomic approaches. In an effort to understand embryonic liver development in zebrafish, in situ hybridization on cryosections of the liver at different embryonic stages with several liver markers was used for anatomical analysis. To further understand liver development, genetic approach and genomic approach were combined to look for genes which are involved in liver organogenesis. Firstly, high throughput whole mount in situ hybridization method was developed to identify zebrafish mutants with liver defects in the forward genetic screen. 71 liver defect mutants were obtained in the two-year forward genetic screening. Secondly, 16 embryonic liver-enriched genes were identified by microarray and further confirmed by northern blot and whole mount in situ hybridization. Thirdly, one novel gene, liver enriched gene 1, leg1, was chosen from these 16 genes for further characterization. In the leg1 deficient morphants, the liver expansion was blocked. Further study of leg1 demonstrated that the role of leg1 played in liver development might be related to I?113p53, a novel isoform of p53 in zebrafish.
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