Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0026130
Title: Genome wide expression profiling reveals suppression of host defence responses during colonisation by neisseria meningitides but not N. lactamica
Authors: Wong H.E.E.
Li M.-S.
Kroll J.S.
Hibberd M.L. 
Langford P.R.
Keywords: bacterial protein
complement component C1s
transcriptome
tumor necrosis factor alpha
autacoid
bacterial protein
complement component C1s
cytokine
transcriptome
tumor necrosis factor alpha
article
bacterial colonization
bacterial genome
bacterial secretion system
controlled study
disease association
down regulation
energy yield
evolutionary adaptation
gene expression profiling
gene expression regulation
gene induction
genetic association
genetic selection
host resistance
human
human cell
immunomodulation
long term exposure
lung alveolus epithelium
meningococcosis
microarray analysis
monolayer culture
Neisseria lactamica
Neisseria meningitidis
nonhuman
nucleotide sequence
species differentiation
upregulation
adaptation
bronchus
cell line
cytology
cytoplasm
energy metabolism
epithelium cell
genetics
genomics
immunity
immunology
metabolism
microbial viability
microbiology
physiology
secretion
species difference
time
Bacteria (microorganisms)
Neisseria lactamica
Neisseria meningitidis
Adaptation, Physiological
Bacterial Proteins
Bronchi
Cell Line
Complement C1s
Cytokines
Cytoplasm
Down-Regulation
Energy Metabolism
Epithelial Cells
Gene Expression Regulation
Genomics
Humans
Immunity
Inflammation Mediators
Microbial Viability
Neisseria lactamica
Neisseria meningitidis
Species Specificity
Time Factors
Transcriptome
Tumor Necrosis Factor-alpha
Issue Date: 2011
Citation: Wong H.E.E., Li M.-S., Kroll J.S., Hibberd M.L., Langford P.R. (2011). Genome wide expression profiling reveals suppression of host defence responses during colonisation by neisseria meningitides but not N. lactamica. PLoS ONE 6 (10) : e26130. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0026130
Abstract: Both Neisseria meningitidis and the closely related bacterium Neisseria lactamica colonise human nasopharyngeal mucosal surface, but only N. meningitidis invades the bloodstream to cause potentially life-threatening meningitis and septicaemia. We have hypothesised that the two neisserial species differentially modulate host respiratory epithelial cell gene expression reflecting their disease potential. Confluent monolayers of 16HBE14 human bronchial epithelial cells were exposed to live and/or dead N. meningitidis (including capsule and pili mutants) and N. lactamica, and their transcriptomes were compared using whole genome microarrays. Changes in expression of selected genes were subsequently validated using Q-RT-PCR and ELISAs. Live N. meningitidis and N. lactamica induced genes involved in host energy production processes suggesting that both bacterial species utilise host resources. N. meningitidis infection was associated with down-regulation of host defence genes. N. lactamica, relative to N. meningitidis, initiates up-regulation of proinflammatory genes. Bacterial secreted proteins alone induced some of the changes observed. The results suggest N. meningitidis and N. lactamica differentially regulate host respiratory epithelial cell gene expression through colonisation and/or protein secretion, and that this may contribute to subsequent clinical outcomes associated with these bacteria. © 2011 Wong et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/162027
ISSN: 19326203
DOI: 10.1371/journal.pone.0026130
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