Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0036435
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dc.titleSusceptibility and response of human blood monocyte subsets to primary dengue virus infection
dc.contributor.authorWong K.L.
dc.contributor.authorChen W.
dc.contributor.authorBalakrishnan T.
dc.contributor.authorToh Y.X.
dc.contributor.authorFink K.
dc.contributor.authorWong S.-C.
dc.date.accessioned2019-11-11T06:39:38Z
dc.date.available2019-11-11T06:39:38Z
dc.date.issued2012
dc.identifier.citationWong K.L., Chen W., Balakrishnan T., Toh Y.X., Fink K., Wong S.-C. (2012). Susceptibility and response of human blood monocyte subsets to primary dengue virus infection. PLoS ONE 7 (5) : e36435. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0036435
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161983
dc.description.abstractHuman blood monocytes play a central role in dengue infections and form the majority of virus infected cells in the blood. Human blood monocytes are heterogeneous and divided into CD16- and CD16+ subsets. Monocyte subsets play distinct roles during disease, but it is not currently known if monocyte subsets differentially contribute to dengue protection and pathogenesis. Here, we compared the susceptibility and response of the human CD16- and CD16+ blood monocyte subsets to primary dengue virus in vitro. We found that both monocyte subsets were equally susceptible to dengue virus (DENV2 NGC), and capable of supporting the initial production of new infective virus particles. Both monocyte subsets produced anti-viral factors, including IFN-?, CXCL10 and TRAIL. However, CD16+ monocytes were the major producers of inflammatory cytokines and chemokines in response to dengue virus, including IL-1?, TNF-?, IL-6, CCL2, 3 and 4. The susceptibility of both monocyte subsets to infection was increased after IL-4 treatment, but this increase was more profound for the CD16+ monocyte subset, particularly at early time points after virus exposure. These findings reveal the differential role that monocyte subsets might play during dengue disease. © 2012 Wong et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectalpha interferon
dc.subjectchemokine
dc.subjectcytokine
dc.subjectgamma interferon inducible protein 10
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectmacrophage inflammatory protein 1alpha
dc.subjectmacrophage inflammatory protein 1beta
dc.subjectmonocyte chemotactic protein 1
dc.subjecttumor necrosis factor alpha
dc.subjecttumor necrosis factor related apoptosis inducing ligand
dc.subjectchemokine
dc.subjectFc receptor
dc.subjectinterleukin 4
dc.subjectarticle
dc.subjectCD16+ monocyte
dc.subjectCD16- monocyte
dc.subjectcell subpopulation
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectdengue
dc.subjectDengue virus
dc.subjectDengue virus 2
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectinfection sensitivity
dc.subjectleukocyte function
dc.subjectmonocyte
dc.subjectpathogenesis
dc.subjectprotection
dc.subjectvirus particle
dc.subjectbiosynthesis
dc.subjectcell survival
dc.subjectcytology
dc.subjectDengue virus
dc.subjectdrug effect
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectsolubility
dc.subjectvirology
dc.subjectDengue virus
dc.subjectCell Survival
dc.subjectChemokines
dc.subjectDengue Virus
dc.subjectHumans
dc.subjectInterleukin-4
dc.subjectMonocytes
dc.subjectReceptors, IgG
dc.subjectSolubility
dc.typeArticle
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.description.doi10.1371/journal.pone.0036435
dc.description.sourcetitlePLoS ONE
dc.description.volume7
dc.description.issue5
dc.description.pagee36435
dc.published.statePublished
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