Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0036435
Title: | Susceptibility and response of human blood monocyte subsets to primary dengue virus infection | Authors: | Wong K.L. Chen W. Balakrishnan T. Toh Y.X. Fink K. Wong S.-C. |
Keywords: | alpha interferon chemokine cytokine gamma interferon inducible protein 10 interleukin 1beta interleukin 6 macrophage inflammatory protein 1alpha macrophage inflammatory protein 1beta monocyte chemotactic protein 1 tumor necrosis factor alpha tumor necrosis factor related apoptosis inducing ligand chemokine Fc receptor interleukin 4 article CD16+ monocyte CD16- monocyte cell subpopulation controlled study cytokine production dengue Dengue virus Dengue virus 2 human human cell in vitro study infection sensitivity leukocyte function monocyte pathogenesis protection virus particle biosynthesis cell survival cytology Dengue virus drug effect metabolism physiology solubility virology Dengue virus Cell Survival Chemokines Dengue Virus Humans Interleukin-4 Monocytes Receptors, IgG Solubility |
Issue Date: | 2012 | Citation: | Wong K.L., Chen W., Balakrishnan T., Toh Y.X., Fink K., Wong S.-C. (2012). Susceptibility and response of human blood monocyte subsets to primary dengue virus infection. PLoS ONE 7 (5) : e36435. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0036435 | Rights: | Attribution 4.0 International | Abstract: | Human blood monocytes play a central role in dengue infections and form the majority of virus infected cells in the blood. Human blood monocytes are heterogeneous and divided into CD16- and CD16+ subsets. Monocyte subsets play distinct roles during disease, but it is not currently known if monocyte subsets differentially contribute to dengue protection and pathogenesis. Here, we compared the susceptibility and response of the human CD16- and CD16+ blood monocyte subsets to primary dengue virus in vitro. We found that both monocyte subsets were equally susceptible to dengue virus (DENV2 NGC), and capable of supporting the initial production of new infective virus particles. Both monocyte subsets produced anti-viral factors, including IFN-?, CXCL10 and TRAIL. However, CD16+ monocytes were the major producers of inflammatory cytokines and chemokines in response to dengue virus, including IL-1?, TNF-?, IL-6, CCL2, 3 and 4. The susceptibility of both monocyte subsets to infection was increased after IL-4 treatment, but this increase was more profound for the CD16+ monocyte subset, particularly at early time points after virus exposure. These findings reveal the differential role that monocyte subsets might play during dengue disease. © 2012 Wong et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161983 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0036435 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1371_journal_pone_0036435.pdf | 826.19 kB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License