Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0036435
Title: Susceptibility and response of human blood monocyte subsets to primary dengue virus infection
Authors: Wong K.L.
Chen W.
Balakrishnan T.
Toh Y.X.
Fink K.
Wong S.-C. 
Keywords: alpha interferon
chemokine
cytokine
gamma interferon inducible protein 10
interleukin 1beta
interleukin 6
macrophage inflammatory protein 1alpha
macrophage inflammatory protein 1beta
monocyte chemotactic protein 1
tumor necrosis factor alpha
tumor necrosis factor related apoptosis inducing ligand
chemokine
Fc receptor
interleukin 4
article
CD16+ monocyte
CD16- monocyte
cell subpopulation
controlled study
cytokine production
dengue
Dengue virus
Dengue virus 2
human
human cell
in vitro study
infection sensitivity
leukocyte function
monocyte
pathogenesis
protection
virus particle
biosynthesis
cell survival
cytology
Dengue virus
drug effect
metabolism
physiology
solubility
virology
Dengue virus
Cell Survival
Chemokines
Dengue Virus
Humans
Interleukin-4
Monocytes
Receptors, IgG
Solubility
Issue Date: 2012
Citation: Wong K.L., Chen W., Balakrishnan T., Toh Y.X., Fink K., Wong S.-C. (2012). Susceptibility and response of human blood monocyte subsets to primary dengue virus infection. PLoS ONE 7 (5) : e36435. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0036435
Abstract: Human blood monocytes play a central role in dengue infections and form the majority of virus infected cells in the blood. Human blood monocytes are heterogeneous and divided into CD16- and CD16+ subsets. Monocyte subsets play distinct roles during disease, but it is not currently known if monocyte subsets differentially contribute to dengue protection and pathogenesis. Here, we compared the susceptibility and response of the human CD16- and CD16+ blood monocyte subsets to primary dengue virus in vitro. We found that both monocyte subsets were equally susceptible to dengue virus (DENV2 NGC), and capable of supporting the initial production of new infective virus particles. Both monocyte subsets produced anti-viral factors, including IFN-?, CXCL10 and TRAIL. However, CD16+ monocytes were the major producers of inflammatory cytokines and chemokines in response to dengue virus, including IL-1?, TNF-?, IL-6, CCL2, 3 and 4. The susceptibility of both monocyte subsets to infection was increased after IL-4 treatment, but this increase was more profound for the CD16+ monocyte subset, particularly at early time points after virus exposure. These findings reveal the differential role that monocyte subsets might play during dengue disease. © 2012 Wong et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161983
ISSN: 19326203
DOI: 10.1371/journal.pone.0036435
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