Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.ppat.1005191
DC Field | Value | |
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dc.title | Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells | |
dc.contributor.author | Burrack K.S. | |
dc.contributor.author | Tan J.J.L. | |
dc.contributor.author | McCarthy M.K. | |
dc.contributor.author | Her Z. | |
dc.contributor.author | Berger J.N. | |
dc.contributor.author | Ng L.F.P. | |
dc.contributor.author | Morrison T.E. | |
dc.date.accessioned | 2019-11-08T08:46:53Z | |
dc.date.available | 2019-11-08T08:46:53Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Burrack K.S., Tan J.J.L., McCarthy M.K., Her Z., Berger J.N., Ng L.F.P., Morrison T.E. (2015). Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells. PLoS Pathogens 11 (10) : e1005191. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1005191 | |
dc.identifier.issn | 15537366 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161931 | |
dc.description.abstract | Arthritogenic alphaviruses, including Ross River virus (RRV) and chikungunya virus (CHIKV), are responsible for explosive epidemics involving millions of cases. These mosquito-transmitted viruses cause inflammation and injury in skeletal muscle and joint tissues that results in debilitating pain. We previously showed that arginase 1 (Arg1) was highly expressed in myeloid cells in the infected and inflamed musculoskeletal tissues of RRV- and CHIKV-infected mice, and specific deletion of Arg1 from myeloid cells resulted in enhanced viral control. Here, we show that Arg1, along with other genes associated with suppressive myeloid cells, is induced in PBMCs isolated from CHIKV-infected patients during the acute phase as well as the chronic phase, and that high Arg1 expression levels were associated with high viral loads and disease severity. Depletion of both CD4 and CD8 T cells from RRV-infected Arg1-deficient mice restored viral loads to levels detected in T cell-depleted wild-type mice. Moreover, Arg1-expressing myeloid cells inhibited virus-specific T cells in the inflamed and infected musculoskeletal tissues, but not lymphoid tissues, following RRV infection in mice, including suppression of interferon-? and CD69 expression. Collectively, these data enhance our understanding of the immune response following arthritogenic alphavirus infection and suggest that immunosuppressive myeloid cells may contribute to the duration or severity of these debilitating infections. ? 2015 Burrack et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | alpha interferon | |
dc.subject | beta interferon | |
dc.subject | C reactive protein | |
dc.subject | gamma interferon inducible protein 10 | |
dc.subject | interleukin 10 | |
dc.subject | interleukin 6 | |
dc.subject | Arg1 protein, mouse | |
dc.subject | arginase | |
dc.subject | arginase I, human | |
dc.subject | Alphavirus infection | |
dc.subject | animal cell | |
dc.subject | animal experiment | |
dc.subject | animal model | |
dc.subject | animal tissue | |
dc.subject | arthritogenic alphaviral infection | |
dc.subject | Article | |
dc.subject | bone marrow cell | |
dc.subject | controlled study | |
dc.subject | flow cytometry | |
dc.subject | gene expression | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | polymerase chain reaction | |
dc.subject | real time polymerase chain reaction | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | RNA isolation | |
dc.subject | T cell depletion | |
dc.subject | T lymphocyte | |
dc.subject | transcription regulation | |
dc.subject | virus immunity | |
dc.subject | virus isolation | |
dc.subject | virus load | |
dc.subject | Western blotting | |
dc.subject | adoptive transfer | |
dc.subject | Alphavirus infection | |
dc.subject | animal | |
dc.subject | bone marrow cell | |
dc.subject | C57BL mouse | |
dc.subject | chikungunya | |
dc.subject | Chikungunya virus | |
dc.subject | human | |
dc.subject | immunology | |
dc.subject | lymphocyte activation | |
dc.subject | Ross River virus | |
dc.subject | Adoptive Transfer | |
dc.subject | Alphavirus Infections | |
dc.subject | Animals | |
dc.subject | Arginase | |
dc.subject | Blotting, Western | |
dc.subject | Chikungunya Fever | |
dc.subject | Chikungunya virus | |
dc.subject | Flow Cytometry | |
dc.subject | Humans | |
dc.subject | Lymphocyte Activation | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Myeloid Cells | |
dc.subject | Polymerase Chain Reaction | |
dc.subject | Ross River virus | |
dc.subject | T-Lymphocytes | |
dc.subject | Viral Load | |
dc.type | Article | |
dc.contributor.department | DEPT OF BIOCHEMISTRY | |
dc.description.doi | 10.1371/journal.ppat.1005191 | |
dc.description.sourcetitle | PLoS Pathogens | |
dc.description.volume | 11 | |
dc.description.issue | 10 | |
dc.description.page | e1005191 | |
Appears in Collections: | Elements Staff Publications |
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