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https://doi.org/10.1371/journal.ppat.1005561
Title: | IL-12p40/IL-10 Producing preCD8α/Clec9A+Dendritic Cells Are Induced in Neonates upon Listeria monocytogenes Infection | Authors: | Torres D. Köhler A. Delbauve S. Caminschi I. Lahoud M.H. Shortman K. Flamand V. |
Keywords: | antigen B7 antigen CD11b antigen CD24 antigen CD86 antigen CD8alpha antigen clec9A antigen gamma interferon glycoprotein p 15095 interleukin 10 interleukin 12p40 interleukin 23 toll like receptor 3 toll like receptor agonist unclassified drug CD8 antigen CD8alpha antigen Clec9a protein, mouse immunoglobulin receptor interleukin 10 interleukin 12p40 lectin transcriptome adult animal cell animal model Article bacterial strain bare lymphocyte syndrome CD8+ T lymphocyte cell proliferation cell protection cell selection cell transfer colony forming unit controlled study dendritic cell enzyme linked immunosorbent assay flow cytometry gene expression profiling immune cell function assay immune response immunization Listeria monocytogenes listeriosis microarray analysis mouse newborn nonhuman oocyte real time polymerase chain reaction survival rate animal antigen presentation biosynthesis C57BL mouse cytology dendritic cell disease model DNA microarray hematopoietic stem cell immunology innate immunity listeriosis lymphocyte activation metabolism transgenic mouse Animals Animals, Newborn Antigen Presentation Antigens, CD8 CD8-Positive T-Lymphocytes Dendritic Cells Disease Models, Animal Enzyme-Linked Immunosorbent Assay Flow Cytometry Hematopoietic Stem Cells Immunity, Innate Interleukin-10 Interleukin-12 Subunit p40 Lectins, C-Type Listeria monocytogenes Listeriosis Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Transgenic Oligonucleotide Array Sequence Analysis Receptors, Immunologic Transcriptome |
Issue Date: | 2016 | Citation: | Torres D., Köhler A., Delbauve S., Caminschi I., Lahoud M.H., Shortman K., Flamand V. (2016). IL-12p40/IL-10 Producing preCD8α/Clec9A+Dendritic Cells Are Induced in Neonates upon Listeria monocytogenes Infection. PLoS Pathogens 12 (4) : e1005561. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1005561 | Rights: | Attribution 4.0 International | Abstract: | Infection by Listeria monocytogenes (Lm) causes serious sepsis and meningitis leading to mortality in neonates. This work explored the ability of CD11chighlineage DCs to induce CD8+T-cell immune protection against Lm in mice before 7 days of life, a period symbolized by the absence of murine IL-12p70-producing CD11chighCD8α+dendritic cells (DCs). We characterized a dominant functional Batf3-dependent precursor of CD11chighDCs that is Clec9A+CD205+CD24+but CD8α-at 3 days of life. After Lm-OVA infection, these pre-DCs that cross-present Ag display the unique ability to produce high levels of IL-12p40 (not IL-12p70 nor IL-23), which enhances OVA-specific CD8+T cell response, and regulatory IL-10 that limits OVA-specific CD8+T cell response. Targeting these neonatal pre-DCs for the first time with a single treatment of anti-Clec9A-OVA antibody in combination with a DC activating agent such as poly(I:C) increased the protection against later exposure to the Lm-OVA strain. Poly(I:C) was shown to induce IL-12p40 production, but not IL-10 by neonatal pre-DCs. In conclusion, we identified a new biologically active precursor of Clec9A+CD8α-DCs, endowed with regulatory properties in early life that represents a valuable target to augment memory responses to vaccines. 2016 Torres et al. | Source Title: | PLoS Pathogens | URI: | https://scholarbank.nus.edu.sg/handle/10635/161917 | ISSN: | 15537366 | DOI: | 10.1371/journal.ppat.1005561 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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