Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0005579
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dc.titleManipulation of cell: Cell contacts and mesoderm suppressing activity direct lineage choice from pluripotent primitive ectoderm-like cells in culture
dc.contributor.authorHughes J.N.
dc.contributor.authorWashington J.M.
dc.contributor.authorZheng Z.
dc.contributor.authorLau X.K.
dc.contributor.authorYap C.
dc.contributor.authorRathjen P.D.
dc.contributor.authorRathjen J.
dc.date.accessioned2019-11-08T00:53:03Z
dc.date.available2019-11-08T00:53:03Z
dc.date.issued2009
dc.identifier.citationHughes J.N., Washington J.M., Zheng Z., Lau X.K., Yap C., Rathjen P.D., Rathjen J. (2009). Manipulation of cell: Cell contacts and mesoderm suppressing activity direct lineage choice from pluripotent primitive ectoderm-like cells in culture. PLoS ONE 4 (5) : e5579. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0005579
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161838
dc.description.abstractIn the mammal, the pluripotent cells of embryo differentiate and commit to either the mesoderm/endoderm lineages or the ectoderm lineage during gastrulation. In culture, the ability to direct lineage choice from pluripotent cells into the mesoderm/endoderm or ectoderm lineages will enable the development of technologies for the formation of highly enriched or homogenous populations of cells. Here we show that manipulation of cell:cell contact and a mesoderm suppressing activity in culture affects the outcome of pluripotent cell differentiation and when both variables are manipulated appropriately they can direct differentiation to either the mesoderm or ectoderm lineage. The disruption of cell:cell contacts and removal of a mesoderm suppressor activity results in the differentiation of pluripotent, primitive ectoderm-like cells to the mesoderm lineage, while maintenance of cell:cell contacts and inclusion, within the culture medium, of a mesoderm suppressing activity results in the formation of near homogenous populations of ectoderm. Understanding the contribution of these variables in lineage choice provides a framework for the development of directed differentiation protocols that result in the formation of specific cell populations from pluripotent cells in culture. � 2009 Hughes et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectanimal cell
dc.subjectarticle
dc.subjectcell activity
dc.subjectcell contact
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcell fate
dc.subjectcell lineage
dc.subjectcell manipulation
dc.subjectcell population
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectectoderm
dc.subjectmesoderm
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpluripotent stem cell
dc.subjectanimal
dc.subjectcell communication
dc.subjectcell line
dc.subjectculture medium
dc.subjectcytology
dc.subjectdrug effect
dc.subjectectoderm
dc.subjectflow cytometry
dc.subjectmesoderm
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectreverse transcription polymerase chain reaction
dc.subjectstem cell
dc.subjectultrafiltration
dc.subjectMammalia
dc.subjectAnimals
dc.subjectCell Communication
dc.subjectCell Differentiation
dc.subjectCell Line
dc.subjectCell Lineage
dc.subjectCulture Media
dc.subjectEctoderm
dc.subjectFlow Cytometry
dc.subjectMesoderm
dc.subjectMice
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectStem Cells
dc.subjectUltrafiltration
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.description.doi10.1371/journal.pone.0005579
dc.description.sourcetitlePLoS ONE
dc.description.volume4
dc.description.issue5
dc.description.pagee5579
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