Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0005579
Title: Manipulation of cell: Cell contacts and mesoderm suppressing activity direct lineage choice from pluripotent primitive ectoderm-like cells in culture
Authors: Hughes J.N.
Washington J.M.
Zheng Z. 
Lau X.K.
Yap C.
Rathjen P.D.
Rathjen J.
Keywords: animal cell
article
cell activity
cell contact
cell culture
cell differentiation
cell fate
cell lineage
cell manipulation
cell population
controlled study
culture medium
ectoderm
mesoderm
mouse
nonhuman
pluripotent stem cell
animal
cell communication
cell line
culture medium
cytology
drug effect
ectoderm
flow cytometry
mesoderm
metabolism
physiology
reverse transcription polymerase chain reaction
stem cell
ultrafiltration
Mammalia
Animals
Cell Communication
Cell Differentiation
Cell Line
Cell Lineage
Culture Media
Ectoderm
Flow Cytometry
Mesoderm
Mice
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
Ultrafiltration
Issue Date: 2009
Citation: Hughes J.N., Washington J.M., Zheng Z., Lau X.K., Yap C., Rathjen P.D., Rathjen J. (2009). Manipulation of cell: Cell contacts and mesoderm suppressing activity direct lineage choice from pluripotent primitive ectoderm-like cells in culture. PLoS ONE 4 (5) : e5579. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0005579
Rights: Attribution 4.0 International
Abstract: In the mammal, the pluripotent cells of embryo differentiate and commit to either the mesoderm/endoderm lineages or the ectoderm lineage during gastrulation. In culture, the ability to direct lineage choice from pluripotent cells into the mesoderm/endoderm or ectoderm lineages will enable the development of technologies for the formation of highly enriched or homogenous populations of cells. Here we show that manipulation of cell:cell contact and a mesoderm suppressing activity in culture affects the outcome of pluripotent cell differentiation and when both variables are manipulated appropriately they can direct differentiation to either the mesoderm or ectoderm lineage. The disruption of cell:cell contacts and removal of a mesoderm suppressor activity results in the differentiation of pluripotent, primitive ectoderm-like cells to the mesoderm lineage, while maintenance of cell:cell contacts and inclusion, within the culture medium, of a mesoderm suppressing activity results in the formation of near homogenous populations of ectoderm. Understanding the contribution of these variables in lineage choice provides a framework for the development of directed differentiation protocols that result in the formation of specific cell populations from pluripotent cells in culture. � 2009 Hughes et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161838
ISSN: 19326203
DOI: 10.1371/journal.pone.0005579
Rights: Attribution 4.0 International
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