Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0008897
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dc.titleTissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation
dc.contributor.authorShindo A.
dc.contributor.authorHara Y.
dc.contributor.authorYamamoto T.S.
dc.contributor.authorOhkura M.
dc.contributor.authorNakai J.
dc.contributor.authorUeno N.
dc.date.accessioned2019-11-07T08:14:45Z
dc.date.available2019-11-07T08:14:45Z
dc.date.issued2010
dc.identifier.citationShindo A., Hara Y., Yamamoto T.S., Ohkura M., Nakai J., Ueno N. (2010). Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS ONE 5 (2) : e8897. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0008897
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161820
dc.description.abstractThe establishment of cell polarity is crucial for embryonic cells to acquire their proper morphologies and functions, because cell alignment and intracellular events are coordinated in tissues during embryogenesis according to the cell polarity. Although much is known about the molecules involved in cell polarization, the direct trigger of the process remains largely obscure. We previously demonstrated that the tissue boundary between the chordamesoderm and lateral mesoderm of Xenopus laevis is important for chordamesodermal cell polarity. Here, we examined the intracellular calcium dynamics during boundary formation between two different tissues. In a combination culture of nodal-induced chordamesodermal explants and a heterogeneous tissue, such as ectoderm or lateral mesoderm, the chordamesodermal cells near the boundary frequently displayed intracellular calcium elevation; this frequency was significantly less when homogeneous explants were used. Inhibition of the intracellular calcium elevation blocked cell polarization in the chordamesodermal explants. We also observed frequent calcium waves near the boundary of the dorsal marginal zone (DMZ) dissected from an early gastrula-stage embryo. Optical sectioning revealed that where heterogeneous explants touched, the chordamesodermal surface formed a wedge with the narrow end tucked under the heterogeneous explant. No such configuration was seen between homogeneous explants. When physical force was exerted against a chordamesodermal explant with a glass needle at an angle similar to that created in the explant, or migrating chordamesodermal cells crawled beneath a silicone block, intracellular calcium elevation was frequent and cell polarization was induced. Finally, we demonstrated that a purinergic receptor, which is implicated in mechano-sensing, is required for such frequent calcium elevation in chordamesoderm and for cell polarization. This study raises the possibility that tissue-tissue interaction generates mechanical forces through cell-cell contact that initiates coordinated cell polarization through a transient increase in intracellular calcium. � 2010 Shindo et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectcalcium
dc.subjectpurinergic P2Y11 receptor
dc.subjectsilicone
dc.subjectcalcium
dc.subjectpurinergic P2 receptor
dc.subjectXenopus protein
dc.subjectarticle
dc.subjectcalcium cell level
dc.subjectcell contact
dc.subjectcell culture
dc.subjectcell polarity
dc.subjectectoderm
dc.subjectforce
dc.subjectgastrulation
dc.subjectmechanical stress
dc.subjectmesoderm
dc.subjectnonhuman
dc.subjectpolarization
dc.subjecttissue interaction
dc.subjectXenopus laevis
dc.subjectanimal
dc.subjectanimal embryo
dc.subjectcell communication
dc.subjectcytology
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectin situ hybridization
dc.subjectmesoderm
dc.subjectmetabolism
dc.subjectnotochord
dc.subjectphysiology
dc.subjectprenatal development
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsomite
dc.subjectXenopus laevis
dc.subjectAnimals
dc.subjectCalcium
dc.subjectCell Communication
dc.subjectCell Polarity
dc.subjectEctoderm
dc.subjectEmbryo, Nonmammalian
dc.subjectGastrulation
dc.subjectGene Expression Regulation, Developmental
dc.subjectIn Situ Hybridization
dc.subjectMesoderm
dc.subjectNotochord
dc.subjectReceptors, Purinergic P2
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSomites
dc.subjectStress, Mechanical
dc.subjectXenopus laevis
dc.subjectXenopus Proteins
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.1371/journal.pone.0008897
dc.description.sourcetitlePLoS ONE
dc.description.volume5
dc.description.issue2
dc.description.pagee8897
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