Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0015374
Title: Matched sizes of activating and inhibitory receptor ligand pairs are required for optimal signal integration by human natural killer cells
Authors: Köhler K.
Xiong S.
Brzostek J. 
Mehrabi M.
Eissmann P.
Harrison A.
Cordoba S.-P.
Oddos S.
Miloserdov V.
Gould K.
Burroughs N.J.
van der Merwe P.A.
Davis D.M.
Keywords: carrier protein
HLA C antigen
killer cell immunoglobulin like receptor 2DL1
natural killer cell receptor NKG2D
protein mica
unclassified drug
HLA antigen class 1
HLA C antigen
hybrid protein
killer cell immunoglobulin like receptor 2DL1
KIR2DL1 protein, human
KLRK1 protein, human
ligand
MHC class I related chain A
MHC class I-related chain A
natural killer cell lectin like receptor subfamily K
photoprotein
animal cell
article
cell activation
cell contact
complex formation
controlled study
human
human cell
immunological synapse
lymphocyte activation
mouse
natural killer cell
nonhuman
protein binding
protein localization
RNA translation
signal transduction
amino acid sequence
cell line
confocal microscopy
genetics
metabolism
molecular genetics
signal transduction
Amino Acid Sequence
Cell Line
Histocompatibility Antigens Class I
HLA-C Antigens
Humans
Killer Cells, Natural
Ligands
Luminescent Proteins
Lymphocyte Activation
Microscopy, Confocal
Molecular Sequence Data
NK Cell Lectin-Like Receptor Subfamily K
Protein Binding
Receptors, KIR2DL1
Recombinant Fusion Proteins
Signal Transduction
Issue Date: 2010
Citation: Köhler K., Xiong S., Brzostek J., Mehrabi M., Eissmann P., Harrison A., Cordoba S.-P., Oddos S., Miloserdov V., Gould K., Burroughs N.J., van der Merwe P.A., Davis D.M. (2010). Matched sizes of activating and inhibitory receptor ligand pairs are required for optimal signal integration by human natural killer cells. PLoS ONE 5 (11) : e15374. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0015374
Rights: Attribution 4.0 International
Abstract: It has been suggested that receptor-ligand complexes segregate or co-localise within immune synapses according to their size, and this is important for receptor signaling. Here, we set out to test the importance of receptor-ligand complex dimensions for immune surveillance of target cells by human Natural Killer (NK) cells. NK cell activation is regulated by integrating signals from activating receptors, such as NKG2D, and inhibitory receptors, such as KIR2DL1. Elongating the NKG2D ligand MICA reduced its ability to trigger NK cell activation. Conversely, elongation of KIR2DL1 ligand HLA-C reduced its ability to inhibit NK cells. Whereas normal-sized HLA-C was most effective at inhibiting activation by normal-length MICA, only elongated HLA-C could inhibit activation by elongated MICA. Moreover, HLA-C and MICA that were matched in size colocaised, whereas HLA-C and MICA that were different in size were segregated. These results demonstrate that receptorligand dimensions are important in NK cell recognition, and suggest that optimal integration of activating and inhibitory receptor signals requires the receptor-ligand complexes to have similar dimensions. © 2010 Köhler et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161801
ISSN: 19326203
DOI: 10.1371/journal.pone.0015374
Rights: Attribution 4.0 International
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