Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0111146
Title: Molecular integrative clustering of asian gastric cell lines revealed two distinct chemosensitivity clusters
Authors: Choong M.L.
Tan S.H.
Tan T.Z. 
Manesh S.
Ngo A.
Yong J.W.Y.
Yang H.H. 
Lee M.A.
Keywords: 2 (2 difluoromethylbenzimidazol 1 yl) 4,6 dimorpholino 1,3,5 triazine
2 (4 hydroxyphenyl) 4 morpholinopyrido[3',2':4,5]furo[3,2 d]pyrimidine
7,8 dihydro 2 [4 (trifluoromethyl)phenyl] 5h thiopyrano[4,3 d]pyrimidin 4 ol
antineoplastic agent
benzyloxycarbonylleucylleucylleucinal
bortezomib
dactolisib
dasatinib
dovitinib
enzyme inhibitor
fluorouracil
gsk 269962a
luminespib
midostaurin
n (2,3 dihydroxypropoxy) 3,4 difluoro 2 (2 fluoro 4 iodoanilino)benzamide
panobinostat
phosphatidylinositol 3 kinase inhibitor
pik 75
proteosome inhibitor
tanespimycin
unclassified drug
protein kinase
Article
Asian
cancer chemotherapy
cancer survival
chemosensitivity
comparative genomic hybridization
controlled study
copy number variation
drug response
gastric cancer cell line
gene dosage
gene expression
gene mutation
genomics
heterozygosity loss
human
human cell
intracellular signaling
mitochondrion
oxidative phosphorylation
stomach cancer
transcription regulation
Asian continental ancestry group
cluster analysis
drug effects
genetics
IC50
mutation
pathology
signal transduction
stomach tumor
tumor cell line
Antineoplastic Agents
Asian Continental Ancestry Group
Cell Line, Tumor
Cluster Analysis
Humans
Inhibitory Concentration 50
Mutation
Protein Kinases
Signal Transduction
Stomach Neoplasms
Issue Date: 2014
Citation: Choong M.L., Tan S.H., Tan T.Z., Manesh S., Ngo A., Yong J.W.Y., Yang H.H., Lee M.A. (2014). Molecular integrative clustering of asian gastric cell lines revealed two distinct chemosensitivity clusters. PLoS ONE 9 (10) : e111146. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0111146
Abstract: Cell lines recapitulate cancer heterogeneity without the presence of interfering tissue found in primary tumor. Their heterogeneous characteristics are reflected in their multiple genetic abnormalities and variable responsiveness to drug treatments. In order to understand the heterogeneity observed in Asian gastric cancers, we have performed array comparative genomic hybridization (aCGH) on 18 Asian gastric cell lines. Hierarchical clustering and single-sample Gene Set Enrichment Analysis were performed on the aCGH data together with public gene expression data of the same cell lines obtained from the Cancer Cell Line Encyclopedia. We found a large amount of genetic aberrations, with some cell lines having 13 fold more aberrations than others. Frequently mutated genes and cellular pathways are identified in these Asian gastric cell lines. The combined analyses of aCGH and expression data demonstrate correlation of gene copy number variations and expression profiles in human gastric cancer cells. The gastric cell lines can be grouped into 2 integrative clusters (ICs). Gastric cells in IC1 are enriched with gene associated with mitochondrial activities and oxidative phosphorylation while cells in IC2 are enriched with genes associated with cell signaling and transcription regulations. The two clusters of cell lines were shown to have distinct responsiveness towards several chemotherapeutics agents such as PI3 K and proteosome inhibitors. Our molecular integrative clustering provides insight into critical genes and pathways that may be responsible for the differences in survival in response to chemotherapy. © 2014 Choong et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161762
ISSN: 19326203
DOI: 10.1371/journal.pone.0111146
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0111146.pdf7.46 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

2
checked on Aug 5, 2020

Page view(s)

82
checked on Aug 6, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.