Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0044453
Title: Aqueous Cytokine Changes Associated with Posner-Schlossman Syndrome with and without Human Cytomegalovirus
Authors: Li J.
Ang M. 
Cheung C.M.G. 
Vania M.
Chan A.S.Y. 
Waduthantri S.
Yang H. 
Chee S.P. 
Keywords: alpha interferon
antibiotic agent
cytokine
gamma interferon
gamma interferon inducible protein 10
granulocyte colony stimulating factor
interleukin 10
interleukin 12
interleukin 13
interleukin 17
interleukin 1beta
interleukin 2
interleukin 4
interleukin 5
interleukin 6
interleukin 7
interleukin 8
macrophage inflammatory protein 1beta
monocyte chemotactic protein 1
tumor necrosis factor alpha
virus DNA
adult
aged
aqueous humor
article
controlled study
Cytomegalovirus
cytomegalovirus infection
disease association
enzyme activity
female
gene dosage
Herpes simplex virus
human
major clinical study
male
posner schlossman syndrome
prospective study
protein analysis
protein expression
protein function
Rubella virus
species differentiation
Toxoplasma
uveitis
Varicella zoster virus
Adult
Aqueous Humor
Cytokines
Cytomegalovirus Infections
Female
Glaucoma
Humans
Iridocyclitis
Male
Middle Aged
Polymerase Chain Reaction
Prospective Studies
Uveitis
Human herpesvirus 3
Human herpesvirus 5
Rubella virus
Simplexvirus
Toxoplasma
Issue Date: 2012
Citation: Li J., Ang M., Cheung C.M.G., Vania M., Chan A.S.Y., Waduthantri S., Yang H., Chee S.P. (2012). Aqueous Cytokine Changes Associated with Posner-Schlossman Syndrome with and without Human Cytomegalovirus. PLoS ONE 7 (9) : e44453. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0044453
Abstract: Aim: To study the differences in aqueous cytokines in Posner-Schlossman Syndrome (PSS) patients with and without human cytomegalovirus (CMV) DNA in the aqueous humor. Methods: This is a prospective study. Fifty-three uveitis patients with clinical signs of PSS were enrolled and aqueous humor samples were collected. Fourteen PSS patients were positive of CMV DNA in the aqueous by polymerase chain reaction (PCR) analysis. These eyes were negative of common ocular pathogens such as herpes simplex virus, varicella-zoster virus, rubella virus and toxoplasma. Twenty-five otherwise healthy cataract patients were enrolled as controls. Cytokine concentration was measured by a magnetic color-bead-based multiplex assay and analyzed using statistical and classification approaches. Results: The average age of 53 PSS patients was 48.74±13.43 years (yrs) (mean ± standard deviation) and 66.3±15.0 yrs for the controls. The median CMV viral DNA copy number was 26000/mL aqueous (range 1400 to 85000 copies/mL) in 14 CMV positive patients as determined by quantitative PCR. PSS aqueous had significantly higher Interleukin (IL)-8 (CXCL8), monocyte chemotactic protein-1 (CCL2), macrophage inhibitory protein 1-? (CCL4), granulocyte colony-stimulating factor (GCSF) and transforming growth factor-? (TGF-?) levels than controls after adjusted by age and gender. IL-2, IL-12, tumor necrosis factor-? (TNF-?) and interferon-? (IFN-?) levels were significantly lower in PSS aqueous than controls. No difference between CMV positive PSS and CMV negative PSS aqueous was observed. Over 97% of PSS samples were distinguished from controls by elevated CXCL10 (>500 ng/mL), CXCL8 (>30 ng/mL) and CCL2 (>60 ng/mL) levels. Conclusion: PSS eyes were characterized by elevated aqueous chemokine concentration. The presence of CMV viral DNA was not associated with significant change of the type of cytokine expression in PSS patients. © 2012 Li et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161732
ISSN: 19326203
DOI: 10.1371/journal.pone.0044453
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0044453.pdf967.54 kBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

20
checked on Mar 28, 2020

Page view(s)

52
checked on Mar 26, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.