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Title: The AHL- and BDSF-Dependent Quorum Sensing Systems Control Specific and Overlapping Sets of Genes in Burkholderia cenocepacia H111
Authors: Schmid N.
Pessi G.
Deng Y.
Aguilar C.
Carlier A.L.
Grunau A.
Omasits U.
Zhang L.-H. 
Ahrens C.H.
Eberl L.
Keywords: bacterial protein
cis 2 dodecenoic acid
messenger RNA
n acylhomoserine lactone
protein BapA
unclassified drug
aidA gene
bacterial gene
bacterial strain
bclA gene
bclB gene
bclC gene
Burkholderia cenocepacia
cepI gene
controlled study
enzyme synthesis
gene control
gene expression regulation
gene mapping
gene mutation
gene targeting
genetic code
quorum sensing
RNA sequence
rpfF gene
type I secretion system
wild type
Burkholderia cenocepacia
Cell Communication
Fatty Acids, Monounsaturated
Gene Expression Regulation, Bacterial
Genes, Bacterial
Peptide Hydrolases
Quorum Sensing
Signal Transduction
Burkholderia cenocepacia
Issue Date: 2012
Citation: Schmid N., Pessi G., Deng Y., Aguilar C., Carlier A.L., Grunau A., Omasits U., Zhang L.-H., Ahrens C.H., Eberl L. (2012). The AHL- and BDSF-Dependent Quorum Sensing Systems Control Specific and Overlapping Sets of Genes in Burkholderia cenocepacia H111. PLoS ONE 7 (11) : e49966. ScholarBank@NUS Repository.
Abstract: Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production. © 2012 Schmid et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0049966
Appears in Collections:Staff Publications

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