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https://doi.org/10.1371/journal.pone.0049966
Title: | The AHL- and BDSF-Dependent Quorum Sensing Systems Control Specific and Overlapping Sets of Genes in Burkholderia cenocepacia H111 | Authors: | Schmid N. Pessi G. Deng Y. Aguilar C. Carlier A.L. Grunau A. Omasits U. Zhang L.-H. Ahrens C.H. Eberl L. |
Keywords: | bacterial protein cis 2 dodecenoic acid hydroxyacid lectin messenger RNA n acylhomoserine lactone protein BapA unclassified drug aidA gene article bacterial gene bacterial strain bclA gene bclB gene bclC gene biofilm Burkholderia cenocepacia cepI gene controlled study enzyme synthesis gene control gene expression regulation gene mapping gene mutation gene targeting genetic code nonhuman operon proteomics quorum sensing regulon RNA sequence rpfF gene stimulon type I secretion system wild type Acyl-Butyrolactones Biofilms Burkholderia cenocepacia Cell Communication Fatty Acids, Monounsaturated Gene Expression Regulation, Bacterial Genes, Bacterial Peptide Hydrolases Quorum Sensing Signal Transduction Burkholderia cenocepacia |
Issue Date: | 2012 | Citation: | Schmid N., Pessi G., Deng Y., Aguilar C., Carlier A.L., Grunau A., Omasits U., Zhang L.-H., Ahrens C.H., Eberl L. (2012). The AHL- and BDSF-Dependent Quorum Sensing Systems Control Specific and Overlapping Sets of Genes in Burkholderia cenocepacia H111. PLoS ONE 7 (11) : e49966. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0049966 | Rights: | Attribution 4.0 International | Abstract: | Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production. © 2012 Schmid et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161705 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0049966 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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