Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pbio.0020095
DC Field | Value | |
---|---|---|
dc.title | A nuclear function for armadillo/?-catenin | |
dc.contributor.author | Tolwinski N.S. | |
dc.contributor.author | Wieschaus E. | |
dc.date.accessioned | 2019-11-06T09:38:58Z | |
dc.date.available | 2019-11-06T09:38:58Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Tolwinski N.S., Wieschaus E. (2004). A nuclear function for armadillo/?-catenin. PLoS Biology 2 (4). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.0020095 | |
dc.identifier.issn | 15449173 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161692 | |
dc.description.abstract | The Wnt signaling pathway provides key information during development of vertebrates and invertebrates, and mutations in this pathway lead to various forms of cancer. Wnt binding to its receptor causes the stabilization and nuclear localization of ?-catenin. Nuclear ?-catenin then functions to activate transcription in conjunction with the transcription factor TCF. A recent report has challenged this basic precept of the Wnt signaling field, arguing that the nuclear localization of ?-catenin may be unrelated to its function and that ?-catenin functions at the plasma membrane to activate this signaling pathway. Here we present evidence that the pathway in fact does depend on the nuclear localization of ?-catenin. We reexamine the functionality of various truncations of ?-catenin and find that only the most severe truncations are true signaling-null mutations. Further, we define a signaling-null condition and use it to show that membrane-tethered ?-catenin is insufficient to activate transcription. We also define two novel loss-of-function mutations that are not truncations, but are missense point mutations that retain protein stability. These alleles allow us to show that the membrane-bound form of activated ?-catenin does indeed depend on the endogenous protein. Further, this activity is dependent on the presence of the C-terminus-specific negative regulator Chibby. Our data clearly show that nuclear localization of ?-catenin is in fact necessary for Wnt pathway activation. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | catenin | |
dc.subject | Wnt protein | |
dc.subject | armadillo domain protein | |
dc.subject | armadillo protein, Drosophila | |
dc.subject | beta catenin | |
dc.subject | carrier protein | |
dc.subject | Chibby protein, Drosophila | |
dc.subject | Drosophila protein | |
dc.subject | nuclear protein | |
dc.subject | transcription factor | |
dc.subject | allele | |
dc.subject | article | |
dc.subject | controlled study | |
dc.subject | Drosophila | |
dc.subject | embryo | |
dc.subject | missense mutation | |
dc.subject | nonhuman | |
dc.subject | nucleotide sequence | |
dc.subject | point mutation | |
dc.subject | protein function | |
dc.subject | protein localization | |
dc.subject | protein stability | |
dc.subject | signal transduction | |
dc.subject | transcription initiation | |
dc.subject | animal | |
dc.subject | cell membrane | |
dc.subject | cell nucleus | |
dc.subject | cross breeding | |
dc.subject | Drosophila melanogaster | |
dc.subject | fluorescence microscopy | |
dc.subject | metabolism | |
dc.subject | molecular genetics | |
dc.subject | mutation | |
dc.subject | phenotype | |
dc.subject | physiology | |
dc.subject | protein tertiary structure | |
dc.subject | transgene | |
dc.subject | Western blotting | |
dc.subject | Armadillo | |
dc.subject | Invertebrata | |
dc.subject | Vertebrata | |
dc.subject | Alleles | |
dc.subject | Animals | |
dc.subject | Armadillo Domain Proteins | |
dc.subject | beta Catenin | |
dc.subject | Blotting, Western | |
dc.subject | Carrier Proteins | |
dc.subject | Cell Membrane | |
dc.subject | Cell Nucleus | |
dc.subject | Crosses, Genetic | |
dc.subject | Drosophila melanogaster | |
dc.subject | Drosophila Proteins | |
dc.subject | Microscopy, Fluorescence | |
dc.subject | Molecular Sequence Data | |
dc.subject | Mutation | |
dc.subject | Mutation, Missense | |
dc.subject | Nuclear Proteins | |
dc.subject | Phenotype | |
dc.subject | Protein Structure, Tertiary | |
dc.subject | Signal Transduction | |
dc.subject | Transcription Factors | |
dc.subject | Transgenes | |
dc.subject | Wnt Proteins | |
dc.type | Article | |
dc.contributor.department | YALE-NUS COLLEGE | |
dc.description.doi | 10.1371/journal.pbio.0020095 | |
dc.description.sourcetitle | PLoS Biology | |
dc.description.volume | 2 | |
dc.description.issue | 4 | |
Appears in Collections: | Elements Staff Publications |
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