Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pbio.0020095
DC FieldValue
dc.titleA nuclear function for armadillo/?-catenin
dc.contributor.authorTolwinski N.S.
dc.contributor.authorWieschaus E.
dc.date.accessioned2019-11-06T09:38:58Z
dc.date.available2019-11-06T09:38:58Z
dc.date.issued2004
dc.identifier.citationTolwinski N.S., Wieschaus E. (2004). A nuclear function for armadillo/?-catenin. PLoS Biology 2 (4). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.0020095
dc.identifier.issn15449173
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161692
dc.description.abstractThe Wnt signaling pathway provides key information during development of vertebrates and invertebrates, and mutations in this pathway lead to various forms of cancer. Wnt binding to its receptor causes the stabilization and nuclear localization of ?-catenin. Nuclear ?-catenin then functions to activate transcription in conjunction with the transcription factor TCF. A recent report has challenged this basic precept of the Wnt signaling field, arguing that the nuclear localization of ?-catenin may be unrelated to its function and that ?-catenin functions at the plasma membrane to activate this signaling pathway. Here we present evidence that the pathway in fact does depend on the nuclear localization of ?-catenin. We reexamine the functionality of various truncations of ?-catenin and find that only the most severe truncations are true signaling-null mutations. Further, we define a signaling-null condition and use it to show that membrane-tethered ?-catenin is insufficient to activate transcription. We also define two novel loss-of-function mutations that are not truncations, but are missense point mutations that retain protein stability. These alleles allow us to show that the membrane-bound form of activated ?-catenin does indeed depend on the endogenous protein. Further, this activity is dependent on the presence of the C-terminus-specific negative regulator Chibby. Our data clearly show that nuclear localization of ?-catenin is in fact necessary for Wnt pathway activation.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectcatenin
dc.subjectWnt protein
dc.subjectarmadillo domain protein
dc.subjectarmadillo protein, Drosophila
dc.subjectbeta catenin
dc.subjectcarrier protein
dc.subjectChibby protein, Drosophila
dc.subjectDrosophila protein
dc.subjectnuclear protein
dc.subjecttranscription factor
dc.subjectallele
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectDrosophila
dc.subjectembryo
dc.subjectmissense mutation
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpoint mutation
dc.subjectprotein function
dc.subjectprotein localization
dc.subjectprotein stability
dc.subjectsignal transduction
dc.subjecttranscription initiation
dc.subjectanimal
dc.subjectcell membrane
dc.subjectcell nucleus
dc.subjectcross breeding
dc.subjectDrosophila melanogaster
dc.subjectfluorescence microscopy
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectmutation
dc.subjectphenotype
dc.subjectphysiology
dc.subjectprotein tertiary structure
dc.subjecttransgene
dc.subjectWestern blotting
dc.subjectArmadillo
dc.subjectInvertebrata
dc.subjectVertebrata
dc.subjectAlleles
dc.subjectAnimals
dc.subjectArmadillo Domain Proteins
dc.subjectbeta Catenin
dc.subjectBlotting, Western
dc.subjectCarrier Proteins
dc.subjectCell Membrane
dc.subjectCell Nucleus
dc.subjectCrosses, Genetic
dc.subjectDrosophila melanogaster
dc.subjectDrosophila Proteins
dc.subjectMicroscopy, Fluorescence
dc.subjectMolecular Sequence Data
dc.subjectMutation
dc.subjectMutation, Missense
dc.subjectNuclear Proteins
dc.subjectPhenotype
dc.subjectProtein Structure, Tertiary
dc.subjectSignal Transduction
dc.subjectTranscription Factors
dc.subjectTransgenes
dc.subjectWnt Proteins
dc.typeArticle
dc.contributor.departmentYALE-NUS COLLEGE
dc.description.doi10.1371/journal.pbio.0020095
dc.description.sourcetitlePLoS Biology
dc.description.volume2
dc.description.issue4
Appears in Collections:Elements
Staff Publications

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pbio_0020095.pdf487.5 kBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons