Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1000131
Title: E1 ubiquitin-activating enzyme UBA-1 plays multiple roles throughout C. elegans development
Authors: Kulkarni M. 
Smith H.E.
Keywords: e1 ubiquitin activating enzyme uba 1
ubiquitin protein ligase
ubiquitin protein ligase E3
4',6 diamidino 2 phenylindole
anaphase promoting complex
anaphase-promoting complex
Caenorhabditis elegans protein
fluorescent dye
indole derivative
ubiquitin
animal cell
animal tissue
article
Caenorhabditis elegans
controlled study
embryo
embryo development
enzyme activity
gene
gene mutation
hermaphroditism
larval development
male
nonhuman
uba 1 gene
ubiquitination
allele
animal
animal embryo
Caenorhabditis elegans
congenital malformation
cytology
enzymology
gene
genetic complementation
genetics
growth, development and aging
heterozygote
homozygote
hydrolysis
meiosis
metabolism
mutation
prenatal development
spermatozoon
Animalia
Caenorhabditis elegans
Metazoa
Alleles
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Embryo, Nonmammalian
Fluorescent Dyes
Genes, Helminth
Genetic Complementation Test
Heterozygote
Homozygote
Hydrolysis
Indoles
Male
Meiosis
Mutation
Spermatozoa
Ubiquitin-Activating Enzymes
Ubiquitin-Protein Ligase Complexes
Ubiquitin-Protein Ligases
Ubiquitination
Ubiquitins
Issue Date: 2008
Citation: Kulkarni M., Smith H.E. (2008). E1 ubiquitin-activating enzyme UBA-1 plays multiple roles throughout C. elegans development. PLoS Genetics 4 (7) : e1000131. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1000131
Rights: Attribution 4.0 International
Abstract: Poly-ubiquitination of target proteins typically marks them for destruction via the proteasome and provides an essential mechanism for the dynamic control of protein levels. The E1 ubiquitin-activating enzyme lies at the apex of the ubiquitination cascade, and its activity is necessary for all subsequent steps in the reaction. We have isolated a temperature-sensitive mutation in the Caenorhabditis elegans uba-1 gene, which encodes the sole E1 enzyme in this organism. Manipulation of UBA-1 activity at different developmental stages reveals a variety of functions for ubiquitination, including novel roles in sperm fertility, control of body size, and sex-specific development. Levels of ubiquitin conjugates are substantially reduced in the mutant, consistent with reduced E1 activity. The uba-1 mutation causes delays in meiotic progression in the early embryo, a process that is known to be regulated by ubiquitin-mediated proteolysis. The uba-1 mutation also demonstrates synthetic lethal interactions with alleles of the anaphase-promoting complex, an E3 ubiquitin ligase. The uba-1 mutation provides a sensitized genetic background for identifying new in vivo functions for downstream components of the ubiquitin enzyme cascade, and it is one of the first conditional mutations reported for the essential E1 enzyme in a metazoan animal model. © 2008 Kulkarni et al.
Source Title: PLoS Genetics
URI: https://scholarbank.nus.edu.sg/handle/10635/161685
ISSN: 15537390
DOI: 10.1371/journal.pgen.1000131
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pgen_1000131.pdf712.74 kBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

37
checked on Mar 6, 2021

Page view(s)

108
checked on Mar 5, 2021

Download(s)

1
checked on Mar 5, 2021

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons