Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.ppat.1000505
Title: Dynamic imaging of CD8+ T cells and dendritic cells during infection with Toxoplasma gondii
Authors: John B.
Harris T.H.
Tait E.D.
Wilson E.H.
Gregg B.
Ng L.G. 
Mrass P.
Roos D.S.
Dzierszinski F.
Weninger W.
Hunter C.A.
Keywords: macrophage inflammatory protein 1alpha
secondary lymphoid tissue chemokine
animal experiment
animal model
article
CD8+ T lymphocyte
cell expansion
dendritic cell
immune response
microscopy
mouse
nonhuman
protein expression
Toxoplasma gondii
toxoplasmosis
analysis of variance
animal
cell motion
flow cytometry
kinetics
lymph node
metabolism
methodology
multiphoton microscopy
parasitology
pathology
physiology
Toxoplasma
transgenic mouse
Toxoplasma gondii
Analysis of Variance
Animals
CD8-Positive T-Lymphocytes
Cell Movement
Dendritic Cells
Flow Cytometry
Kinetics
Lymph Nodes
Mice
Mice, Transgenic
Microscopy, Fluorescence, Multiphoton
Toxoplasma
Toxoplasmosis
Issue Date: 2009
Citation: John B., Harris T.H., Tait E.D., Wilson E.H., Gregg B., Ng L.G., Mrass P., Roos D.S., Dzierszinski F., Weninger W., Hunter C.A. (2009). Dynamic imaging of CD8+ T cells and dendritic cells during infection with Toxoplasma gondii. PLoS Pathogens 5 (7) : e1000505. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1000505
Rights: Attribution 4.0 International
Abstract: To better understand the initiation of CD8+ T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite specific CD8+ T cells. Infection with T. gondii induced localization of both these populations to the sub-capsular/interfollicular region of the draining lymph node and DCs were required for the expansion of the T cells. Consistent with current models, in the presence of cognate antigen, the average velocity of CD8+ T cells decreased. Unexpectedly, infection also resulted in modulation of the behavior of non-parasite specific T cells. This TCR-independent process correlated with the re-modeling of the lymph node micro-architecture and changes in expression of CCL21 and CCL3. Infection also resulted in sustained interactions between the DCs and CD8+ T cells that were visualized only in the presence of cognate antigen and were limited to an early phase in the response. Infected DCs were rare within the lymph node during this time frame; however, DCs presenting the cognate antigen were detected. Together, these data provide novel insights into the earliest interaction between DCs and CD8+ T cells and suggest that cross presentation by bystander DCs rather than infected DCs is an important route of antigen presentation during toxoplasmosis. © 2009 John et al.
Source Title: PLoS Pathogens
URI: https://scholarbank.nus.edu.sg/handle/10635/161672
ISSN: 15537366
DOI: 10.1371/journal.ppat.1000505
Rights: Attribution 4.0 International
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